Whole Genome DNA Methylation and Mutational Profiles Identify Novel Changes in Proliferative Verrucous Leukoplakia

Eyituoyo M Okoturo, Daniel Green, Kim Clarke, Triantafyllos Liloglou, Mark T Boyd, Richard J Shaw, Janet M Risk

Research output: Contribution to journalArticle (journal)peer-review

3 Citations (Scopus)
22 Downloads (Pure)

Abstract

Proliferative verrucous leukoplakia (PVL) is a rare form of oral leukoplakia with a relatively high transformation rate resulting in oral squamous cell carcinoma (OSCC). Molecular analysis of PVL at the genome level is limited and has only identified molecular similarities between PVL and OSCC. However, the clinical profile of PVL suggests that molecular differences may be more important. Whole exome sequencing of 5 PVL-associated OSCC (PVL-OSCC) and paired blood samples was used to identify somatic mutations common to the tumors. Whole methylome analysis of samples from 4 PVL-associated OSCC and 3 OSCC of non-PVL origin samples was conducted to explore differential methylation. In contrast to conventional OSCC, PVL-associated OSCC showed infrequent TP53 mutation and altered spectra of PIK3CA and NOTCH1 mutations. Unsupervised hierarchical clustering identified 63 probes that discriminated between PVL-associated OSCC and OSCC of non-PVL origin. Differences in methylation were most significant for divalent metal ion transport, particularly calcium movement. Specific differences in mutation and methylation profiles between PVL-derived OSCC and OSCC of non-PVL origin suggest differences in their transformation pathways. Further studies of early PVL lesions may identify markers of transformation that are also applicable to more common oral premalignant disorders such as oral epithelial dysplasia. [Abstract copyright: Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.]
Original languageEnglish
Pages (from-to)893-903
Number of pages11
JournalOral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
Volume135
Issue number6
Early online date17 Mar 2023
DOIs
Publication statusPublished - 1 Jun 2023

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