Urinary proteomic profiling in severe obesity and obstructive sleep apnoea with CPAP treatment

Ian Seetho, Adela Ramirez-Torres, Amaya Albalat, William Mullen , Harald Mischak, Robert Parker, Sonya Craig, KEVIN HARDY, Nick Duffy, Kevin Hardy , Jatin Burniston, John Wilding

Research output: Contribution to journalArticle

3 Citations (Scopus)
68 Downloads (Pure)

Abstract

Introduction: Obstructive sleep apnoea (OSA) is common in obesity and is associated with cardiovascular and metabolic complications. Continuous positive airway pressure (CPAP) in OSA may lead to physiological changes reflected in the urinary proteome. The aim of this study was to characterise the urinary proteome in severely obese adult subjects with OSA who were receiving CPAP compared with severely obese subjects without OSA. Methods: Severely obese subjects with and without OSA were recruited. Subjects with OSA were receiving CPAP. Body composition and blood pressure measurements were recorded. Urinary samples were analysed by Capillary Electrophoresis-Mass Spectrometry (CE-MS). Results: Twenty-seven subjects with OSA-on-CPAP (age 49 + 7years, BMI 43 + 7kg/m 2 ) and 25 controls without OSA (age 52+9years, BMI 39+4kg/m 2 ) were studied. Age and BMI were not significantly different between groups. Mean CPAP use for OSA patients was 14.5 + 1.0 months. Metabolic syndrome was present in 14(52%) of those with OSA compared with 6(24%) of controls (p=0.039). A urinary proteome comprising 15 peptides was identified showing differential expression between the groups (p<0.01). Although correction for multiple testing did not reach significance, sequences were determined for 8 peptides demonstrating origins from collagens, fibrinogen beta chain and T-cadherin that may be associated with underlying cardiovascular disease mechanisms in OSA. Conclusions: The urinary proteome is compared in OSA with CPAP and without OSA in severe obesity. The effects of CPAP on OSA may lead to changes in the urinary peptides but further research work is needed to investigate the potential role for urinary proteomics in characteris­ing urinary peptide profiles in OSA.

Original languageEnglish
Pages (from-to)58-67
Number of pages10
JournalSleep Science
Volume8
Issue number2
Early online date17 Jul 2015
DOIs
Publication statusE-pub ahead of print - 17 Jul 2015

Keywords

  • Obstructive sleep apnoea
  • Severe obesity
  • Urinary proteomics
  • CPAP

Fingerprint Dive into the research topics of 'Urinary proteomic profiling in severe obesity and obstructive sleep apnoea with CPAP treatment'. Together they form a unique fingerprint.

  • Cite this

    Seetho, I., Ramirez-Torres, A., Albalat, A., Mullen , W., Mischak, H., Parker, R., Craig, S., HARDY, KEVIN., Duffy, N., Hardy , K., Burniston, J., & Wilding, J. (2015). Urinary proteomic profiling in severe obesity and obstructive sleep apnoea with CPAP treatment. Sleep Science, 8(2), 58-67. https://doi.org/10.1016/j.slsci.2015.06.004