Underpinning Sustainable Vector Control through Informed Insecticide Resistance Management

Edward K. Thomsen, Clare Strode, Kay Hemmings, Angela J. Hughes, Emmanuel Chanda, Mulenga Musapa, Mulakwa Kamuliwo, Faustina N. Phiri, Lucy Muzia, Javan Chanda, Alister Kandyata, Brian Chirwa, Kathleen Poer, Janet Hemingway, Charles S. Wondji, Hilary Ranson, Michael Coleman

Research output: Contribution to journalArticle

30 Citations (Scopus)
9 Downloads (Pure)

Abstract

Background: There has been rapid scale-up of malaria vector control in the last ten years. Both of the primary control strategies, long-lasting pyrethroid treated nets and indoor residual spraying, rely on the use of a limited number of insecticides. Insecticide resistance, as measured by bioassay, has rapidly increased in prevalence and has come to the forefront as an issue that needs to be addressed to maintain the sustainability of malaria control and the drive to elimination. Zambia’s programme reported high levels of resistance to the insecticides it used in 2010, and, as a result, increased its investment in resistance monitoring to support informed resistance management decisions. Methodology/Principal Findings: A country-wide survey on insecticide resistance in Zambian malaria vectors was performed using WHO bioassays to detect resistant phenotypes. Molecular techniques were used to detect target-site mutations and microarray to detect metabolic resistance mechanisms. Anopheles gambiae s.s. was resistant to pyrethroids, DDT and carbamates, with potential organophosphate resistance in one population. The resistant phenotypes were conferred by both target-site and metabolic mechanisms. Anopheles funestus s.s. was largely resistant to pyrethroids and carbamates, with potential resistance to DDT in two locations. The resistant phenotypes were conferred by elevated levels of cytochrome p450s. Conclusions/Significance: Currently, the Zambia National Malaria Control Centre is using these results to inform their vector control strategy. The methods employed here can serve as a template to all malaria-endemic countries striving to create a sustainable insecticide resistance management plan
Original languageEnglish
Pages (from-to)1-13
JournalPLoS ONE
Volume9
Issue number6
Early online date16 Jun 2014
DOIs
Publication statusE-pub ahead of print - 16 Jun 2014

Fingerprint

Insecticide Resistance
resistance management
vector control
insecticide resistance
Insecticides
Pyrethrins
malaria
Malaria
Malaria control
pyrethrins
DDT
Carbamates
Bioassay
DDT (pesticide)
carbamates
Phenotype
phenotype
Biological Assay
bioassays
Anopheles funestus

Cite this

Thomsen, E. K., Strode, C., Hemmings, K., Hughes, A. J., Chanda, E., Musapa, M., ... Coleman, M. (2014). Underpinning Sustainable Vector Control through Informed Insecticide Resistance Management. PLoS ONE, 9(6), 1-13. https://doi.org/doi:10.1371/journal.pone.0099822
Thomsen, Edward K. ; Strode, Clare ; Hemmings, Kay ; Hughes, Angela J. ; Chanda, Emmanuel ; Musapa, Mulenga ; Kamuliwo, Mulakwa ; Phiri, Faustina N. ; Muzia, Lucy ; Chanda, Javan ; Kandyata, Alister ; Chirwa, Brian ; Poer, Kathleen ; Hemingway, Janet ; Wondji, Charles S. ; Ranson, Hilary ; Coleman, Michael. / Underpinning Sustainable Vector Control through Informed Insecticide Resistance Management. In: PLoS ONE. 2014 ; Vol. 9, No. 6. pp. 1-13.
@article{0e58e1a8e3ba40e5803c90d03a7befd5,
title = "Underpinning Sustainable Vector Control through Informed Insecticide Resistance Management",
abstract = "Background: There has been rapid scale-up of malaria vector control in the last ten years. Both of the primary control strategies, long-lasting pyrethroid treated nets and indoor residual spraying, rely on the use of a limited number of insecticides. Insecticide resistance, as measured by bioassay, has rapidly increased in prevalence and has come to the forefront as an issue that needs to be addressed to maintain the sustainability of malaria control and the drive to elimination. Zambia’s programme reported high levels of resistance to the insecticides it used in 2010, and, as a result, increased its investment in resistance monitoring to support informed resistance management decisions. Methodology/Principal Findings: A country-wide survey on insecticide resistance in Zambian malaria vectors was performed using WHO bioassays to detect resistant phenotypes. Molecular techniques were used to detect target-site mutations and microarray to detect metabolic resistance mechanisms. Anopheles gambiae s.s. was resistant to pyrethroids, DDT and carbamates, with potential organophosphate resistance in one population. The resistant phenotypes were conferred by both target-site and metabolic mechanisms. Anopheles funestus s.s. was largely resistant to pyrethroids and carbamates, with potential resistance to DDT in two locations. The resistant phenotypes were conferred by elevated levels of cytochrome p450s. Conclusions/Significance: Currently, the Zambia National Malaria Control Centre is using these results to inform their vector control strategy. The methods employed here can serve as a template to all malaria-endemic countries striving to create a sustainable insecticide resistance management plan",
author = "Thomsen, {Edward K.} and Clare Strode and Kay Hemmings and Hughes, {Angela J.} and Emmanuel Chanda and Mulenga Musapa and Mulakwa Kamuliwo and Phiri, {Faustina N.} and Lucy Muzia and Javan Chanda and Alister Kandyata and Brian Chirwa and Kathleen Poer and Janet Hemingway and Wondji, {Charles S.} and Hilary Ranson and Michael Coleman",
note = "e99822",
year = "2014",
month = "6",
day = "16",
doi = "doi:10.1371/journal.pone.0099822",
language = "English",
volume = "9",
pages = "1--13",
journal = "PLoS ONE",
issn = "1932-6203",
publisher = "PLOS",
number = "6",

}

Thomsen, EK, Strode, C, Hemmings, K, Hughes, AJ, Chanda, E, Musapa, M, Kamuliwo, M, Phiri, FN, Muzia, L, Chanda, J, Kandyata, A, Chirwa, B, Poer, K, Hemingway, J, Wondji, CS, Ranson, H & Coleman, M 2014, 'Underpinning Sustainable Vector Control through Informed Insecticide Resistance Management', PLoS ONE, vol. 9, no. 6, pp. 1-13. https://doi.org/doi:10.1371/journal.pone.0099822

Underpinning Sustainable Vector Control through Informed Insecticide Resistance Management. / Thomsen, Edward K.; Strode, Clare; Hemmings, Kay; Hughes, Angela J.; Chanda, Emmanuel; Musapa, Mulenga; Kamuliwo, Mulakwa; Phiri, Faustina N.; Muzia, Lucy; Chanda, Javan; Kandyata, Alister; Chirwa, Brian; Poer, Kathleen; Hemingway, Janet; Wondji, Charles S.; Ranson, Hilary; Coleman, Michael.

In: PLoS ONE, Vol. 9, No. 6, 16.06.2014, p. 1-13.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Underpinning Sustainable Vector Control through Informed Insecticide Resistance Management

AU - Thomsen, Edward K.

AU - Strode, Clare

AU - Hemmings, Kay

AU - Hughes, Angela J.

AU - Chanda, Emmanuel

AU - Musapa, Mulenga

AU - Kamuliwo, Mulakwa

AU - Phiri, Faustina N.

AU - Muzia, Lucy

AU - Chanda, Javan

AU - Kandyata, Alister

AU - Chirwa, Brian

AU - Poer, Kathleen

AU - Hemingway, Janet

AU - Wondji, Charles S.

AU - Ranson, Hilary

AU - Coleman, Michael

N1 - e99822

PY - 2014/6/16

Y1 - 2014/6/16

N2 - Background: There has been rapid scale-up of malaria vector control in the last ten years. Both of the primary control strategies, long-lasting pyrethroid treated nets and indoor residual spraying, rely on the use of a limited number of insecticides. Insecticide resistance, as measured by bioassay, has rapidly increased in prevalence and has come to the forefront as an issue that needs to be addressed to maintain the sustainability of malaria control and the drive to elimination. Zambia’s programme reported high levels of resistance to the insecticides it used in 2010, and, as a result, increased its investment in resistance monitoring to support informed resistance management decisions. Methodology/Principal Findings: A country-wide survey on insecticide resistance in Zambian malaria vectors was performed using WHO bioassays to detect resistant phenotypes. Molecular techniques were used to detect target-site mutations and microarray to detect metabolic resistance mechanisms. Anopheles gambiae s.s. was resistant to pyrethroids, DDT and carbamates, with potential organophosphate resistance in one population. The resistant phenotypes were conferred by both target-site and metabolic mechanisms. Anopheles funestus s.s. was largely resistant to pyrethroids and carbamates, with potential resistance to DDT in two locations. The resistant phenotypes were conferred by elevated levels of cytochrome p450s. Conclusions/Significance: Currently, the Zambia National Malaria Control Centre is using these results to inform their vector control strategy. The methods employed here can serve as a template to all malaria-endemic countries striving to create a sustainable insecticide resistance management plan

AB - Background: There has been rapid scale-up of malaria vector control in the last ten years. Both of the primary control strategies, long-lasting pyrethroid treated nets and indoor residual spraying, rely on the use of a limited number of insecticides. Insecticide resistance, as measured by bioassay, has rapidly increased in prevalence and has come to the forefront as an issue that needs to be addressed to maintain the sustainability of malaria control and the drive to elimination. Zambia’s programme reported high levels of resistance to the insecticides it used in 2010, and, as a result, increased its investment in resistance monitoring to support informed resistance management decisions. Methodology/Principal Findings: A country-wide survey on insecticide resistance in Zambian malaria vectors was performed using WHO bioassays to detect resistant phenotypes. Molecular techniques were used to detect target-site mutations and microarray to detect metabolic resistance mechanisms. Anopheles gambiae s.s. was resistant to pyrethroids, DDT and carbamates, with potential organophosphate resistance in one population. The resistant phenotypes were conferred by both target-site and metabolic mechanisms. Anopheles funestus s.s. was largely resistant to pyrethroids and carbamates, with potential resistance to DDT in two locations. The resistant phenotypes were conferred by elevated levels of cytochrome p450s. Conclusions/Significance: Currently, the Zambia National Malaria Control Centre is using these results to inform their vector control strategy. The methods employed here can serve as a template to all malaria-endemic countries striving to create a sustainable insecticide resistance management plan

U2 - doi:10.1371/journal.pone.0099822

DO - doi:10.1371/journal.pone.0099822

M3 - Article

VL - 9

SP - 1

EP - 13

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 6

ER -