The RNA Polymerase Omega Factor RpoZ Is Regulated by PhoP and Has an Important Role in Antibiotic Biosynthesis and Morphological Differentiation in Streptomyces coelicolor

F. Santos-Beneit, M. Barriuso-Iglesias, L. T. Fernandez-Martinez, M. Martinez-Castro, A. Sola-Landa, A. Rodriguez-Garcia, J. F. Martin

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Abstract

The RNA polymerase (RNAP) omega factor (ω) forms a complex with the α2ββ′ core of this enzyme in bacteria. We have characterized the rpoZ gene of Streptomyces coelicolor, which encodes a small protein (90 amino acids) identified as the omega factor. Deletion of the rpoZ gene resulted in strains with a slightly reduced growth rate, although they were still able to sporulate. The biosynthesis of actinorhodin and, particularly, that of undecylprodigiosin were drastically reduced in the ΔrpoZ strain, suggesting that expression of these secondary metabolite biosynthetic genes is dependent upon the presence of RpoZ in the RNAP complex. Complementation of the ΔrpoZ mutant with the wild-type rpoZ allele restored both phenotype and antibiotic production. Interestingly, the rpoZ gene contains a PHO box in its promoter region. DNA binding assays showed that the phosphate response regulator PhoP binds to such a region. Since luciferase reporter studies showed that rpoZ promoter activity was increased in a ΔphoP background, it can be concluded that rpoZ is controlled negatively by PhoP, thus connecting phosphate depletion regulation with antibiotic production and morphological differentiation in Streptomyces.
Original languageEnglish
Pages (from-to)7586-7594
JournalApplied and Environmental Microbiology
Volume77
Issue number21
DOIs
Publication statusPublished - 2011

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Streptomyces coelicolor
DNA-Directed RNA Polymerases
DNA-directed RNA polymerase
antibiotics
RNA
biosynthesis
Anti-Bacterial Agents
gene
Phosphates
promoter regions
phosphates
Genes
genes
gene deletion
Streptomyces
Gene Deletion
phosphate
luciferase
Luciferases
Genetic Promoter Regions

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Santos-Beneit, F. ; Barriuso-Iglesias, M. ; Fernandez-Martinez, L. T. ; Martinez-Castro, M. ; Sola-Landa, A. ; Rodriguez-Garcia, A. ; Martin, J. F. / The RNA Polymerase Omega Factor RpoZ Is Regulated by PhoP and Has an Important Role in Antibiotic Biosynthesis and Morphological Differentiation in Streptomyces coelicolor. In: Applied and Environmental Microbiology. 2011 ; Vol. 77, No. 21. pp. 7586-7594.
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The RNA Polymerase Omega Factor RpoZ Is Regulated by PhoP and Has an Important Role in Antibiotic Biosynthesis and Morphological Differentiation in Streptomyces coelicolor. / Santos-Beneit, F.; Barriuso-Iglesias, M.; Fernandez-Martinez, L. T.; Martinez-Castro, M.; Sola-Landa, A.; Rodriguez-Garcia, A.; Martin, J. F.

In: Applied and Environmental Microbiology, Vol. 77, No. 21, 2011, p. 7586-7594.

Research output: Contribution to journalArticle

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AU - Santos-Beneit, F.

AU - Barriuso-Iglesias, M.

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AU - Martin, J. F.

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AB - The RNA polymerase (RNAP) omega factor (ω) forms a complex with the α2ββ′ core of this enzyme in bacteria. We have characterized the rpoZ gene of Streptomyces coelicolor, which encodes a small protein (90 amino acids) identified as the omega factor. Deletion of the rpoZ gene resulted in strains with a slightly reduced growth rate, although they were still able to sporulate. The biosynthesis of actinorhodin and, particularly, that of undecylprodigiosin were drastically reduced in the ΔrpoZ strain, suggesting that expression of these secondary metabolite biosynthetic genes is dependent upon the presence of RpoZ in the RNAP complex. Complementation of the ΔrpoZ mutant with the wild-type rpoZ allele restored both phenotype and antibiotic production. Interestingly, the rpoZ gene contains a PHO box in its promoter region. DNA binding assays showed that the phosphate response regulator PhoP binds to such a region. Since luciferase reporter studies showed that rpoZ promoter activity was increased in a ΔphoP background, it can be concluded that rpoZ is controlled negatively by PhoP, thus connecting phosphate depletion regulation with antibiotic production and morphological differentiation in Streptomyces.

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