TY - JOUR
T1 - The mitochondrial genome of Brachycephalus brunneus (Anura
T2 - Brachycephalidae), with comments on the phylogenetic position of Brachycephalidae
AU - Pie, Marcio R.
AU - Ströher, Patrícia R.
AU - Bornschein, Marcos R.
AU - Ribeiro, Luiz F.
AU - Faircloth, Brant C.
AU - McCormack, John E.
N1 - Funding Information:
We thank W. Tsai for assistance during laboratory work. Samples were collected under ICMBIO permit #22470-2 and Instituto Ambiental do Paran? permit 355/11. This study was funded by the Funda??o Grupo Botic?rio de Prote??o ? Natureza (grants 0895_20111 and A0010_2014), as well as a grant from CNPq to MRP (grant 571334/2008-3).
Publisher Copyright:
© 2017 Elsevier Ltd
PY - 2017/4/1
Y1 - 2017/4/1
N2 - The mitochondrial genome of Brachycephalus brunneus was determined by next-generation sequencing of mitochondrial DNA. Without its control region, it has a total length of 15,485 bp, consisting of 37 genes: 13 protein-coding genes, 2 rRNA genes, and 22 tRNA genes. Except for eight tRNAs and the nd6 gene, all other mitochondrial genes are encoded on the heavy strand. ATG and ATC act mainly as the initial codon in 10 protein-coding genes, whereas nd2 and cox1 use ATT and nad3 uses ATA. Gene order is generally consistent with that observed in closely-related families. The cloverleaf structures for trnS1 and trnC lacked the DHU-stem and DHU-loop, respectively. Phylogenetic analyses of mitogenomes of closely-related families indicate that Brachycephalidae is more closely-related to Craugastoridae than to Eleutherodactylidae. This is the first sequenced mitochondrial genome for the entire Brachycephalidae and can provide the basis for the development of mitochondrial markers for other members of the family, including many species that are critically endangered.
AB - The mitochondrial genome of Brachycephalus brunneus was determined by next-generation sequencing of mitochondrial DNA. Without its control region, it has a total length of 15,485 bp, consisting of 37 genes: 13 protein-coding genes, 2 rRNA genes, and 22 tRNA genes. Except for eight tRNAs and the nd6 gene, all other mitochondrial genes are encoded on the heavy strand. ATG and ATC act mainly as the initial codon in 10 protein-coding genes, whereas nd2 and cox1 use ATT and nad3 uses ATA. Gene order is generally consistent with that observed in closely-related families. The cloverleaf structures for trnS1 and trnC lacked the DHU-stem and DHU-loop, respectively. Phylogenetic analyses of mitogenomes of closely-related families indicate that Brachycephalidae is more closely-related to Craugastoridae than to Eleutherodactylidae. This is the first sequenced mitochondrial genome for the entire Brachycephalidae and can provide the basis for the development of mitochondrial markers for other members of the family, including many species that are critically endangered.
KW - Anuran
KW - Atlantic forest
KW - Brazil
KW - Terrarana
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U2 - 10.1016/j.bse.2016.12.009
DO - 10.1016/j.bse.2016.12.009
M3 - Article (journal)
AN - SCOPUS:85010465156
SN - 0305-1978
VL - 71
SP - 26
EP - 31
JO - Biochemical Systematics and Ecology
JF - Biochemical Systematics and Ecology
ER -