The Anopheles gambiae detoxification chip: a highly specific microarray to study metabolic-based insecticide resistance in malaria vectors.

Jean-Philippe David, Clare Strode, John Vontas, Dimitra Nikou, Ashley Vaughan, Patricia M Pignatelli, Christos Louis, Janet Hemingway, Hilary Ranson

Research output: Contribution to journalArticle

226 Citations (Scopus)

Abstract

Metabolic pathways play an important role in insecticide resistance, but the full spectra of the genes involved in resistance has not been established. We constructed a microarray containing unique fragments from 230 Anopheles gambiae genes putatively involved in insecticide metabolism [cytochrome P450s (P450s), GSTs, and carboxylesterases and redox genes, partners of the P450 oxidative metabolic complex, and various controls]. We used this detox chip to monitor the expression of the detoxifying genes in insecticide resistant and susceptible An. gambiae laboratory strains. Five genes were strongly up-regulated in the dichlorodiphenyltrichloroethane-resistant strain ZAN/U. These genes included the GST GSTE2, which has previously been implicated in dichlorodiphenyltrichloroethane resistance, two P450s, and two peroxidase genes. GSTE2 was also elevated in the pyrethroid-resistant RSP strain. In addition, the P450 CYP325A3, belonging to a class not previously associated with insecticide resistance, was expressed at statistically higher levels in this strain. The applications of this detox chip and its potential contribution to malaria vector insecticide resistance management programs are discussed.
Original languageEnglish
Pages (from-to)4080-4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume102
Issue number11
DOIs
Publication statusPublished - 15 Mar 2005

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metabolic studies
Anopheles gambiae
insecticide resistance
malaria
genes
DDT (pesticide)
insecticides
carboxylesterase
resistance management
cytochromes
pyrethrins
biochemical pathways
peroxidase
gene expression
metabolism

Cite this

David, Jean-Philippe ; Strode, Clare ; Vontas, John ; Nikou, Dimitra ; Vaughan, Ashley ; Pignatelli, Patricia M ; Louis, Christos ; Hemingway, Janet ; Ranson, Hilary. / The Anopheles gambiae detoxification chip: a highly specific microarray to study metabolic-based insecticide resistance in malaria vectors. In: Proceedings of the National Academy of Sciences of the United States of America. 2005 ; Vol. 102, No. 11. pp. 4080-4.
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abstract = "Metabolic pathways play an important role in insecticide resistance, but the full spectra of the genes involved in resistance has not been established. We constructed a microarray containing unique fragments from 230 Anopheles gambiae genes putatively involved in insecticide metabolism [cytochrome P450s (P450s), GSTs, and carboxylesterases and redox genes, partners of the P450 oxidative metabolic complex, and various controls]. We used this detox chip to monitor the expression of the detoxifying genes in insecticide resistant and susceptible An. gambiae laboratory strains. Five genes were strongly up-regulated in the dichlorodiphenyltrichloroethane-resistant strain ZAN/U. These genes included the GST GSTE2, which has previously been implicated in dichlorodiphenyltrichloroethane resistance, two P450s, and two peroxidase genes. GSTE2 was also elevated in the pyrethroid-resistant RSP strain. In addition, the P450 CYP325A3, belonging to a class not previously associated with insecticide resistance, was expressed at statistically higher levels in this strain. The applications of this detox chip and its potential contribution to malaria vector insecticide resistance management programs are discussed.",
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The Anopheles gambiae detoxification chip: a highly specific microarray to study metabolic-based insecticide resistance in malaria vectors. / David, Jean-Philippe; Strode, Clare; Vontas, John; Nikou, Dimitra; Vaughan, Ashley; Pignatelli, Patricia M; Louis, Christos; Hemingway, Janet; Ranson, Hilary.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, No. 11, 15.03.2005, p. 4080-4.

Research output: Contribution to journalArticle

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T1 - The Anopheles gambiae detoxification chip: a highly specific microarray to study metabolic-based insecticide resistance in malaria vectors.

AU - David, Jean-Philippe

AU - Strode, Clare

AU - Vontas, John

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AU - Vaughan, Ashley

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AU - Louis, Christos

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AU - Ranson, Hilary

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