TY - JOUR
T1 - Symbiont-mediated RNA interference in insects
AU - Whitten, Miranda M. A.
AU - Facey, Paul D.
AU - Del Sol, Ricardo
AU - Fernández-Martínez, Lorena T.
AU - Evans, Meirwyn C.
AU - Mitchell, Jacob J.
AU - Bodger, Owen G.
AU - Dyson, Paul J.
PY - 2016/2/24
Y1 - 2016/2/24
N2 - RNA interference (RNAi) methods for insects are often limited by problems
with double-stranded (ds) RNA delivery, which restricts reverse genetics
studies and the development of RNAi-based biocides.We therefore delegated
to insect symbiotic bacteria the task of: (i) constitutive dsRNA synthesis and
(ii) trauma-free delivery. RNaseIII-deficient, dsRNA-expressing bacterial
strains were created from the symbionts of two very diverse pest species: a
long-lived blood-sucking bug, Rhodnius prolixus, and a short-lived globally
invasive polyphagous agricultural pest, western flower thrips (Frankliniella
occidentalis). When ingested, the manipulated bacteria colonized the insects,
successfully competed with the wild-type microflora, and sustainably
mediated systemic knockdown phenotypes that were horizontally transmissible.
This represents a significant advance in the ability to deliver RNAi,
potentially to a large range of non-model insects.
AB - RNA interference (RNAi) methods for insects are often limited by problems
with double-stranded (ds) RNA delivery, which restricts reverse genetics
studies and the development of RNAi-based biocides.We therefore delegated
to insect symbiotic bacteria the task of: (i) constitutive dsRNA synthesis and
(ii) trauma-free delivery. RNaseIII-deficient, dsRNA-expressing bacterial
strains were created from the symbionts of two very diverse pest species: a
long-lived blood-sucking bug, Rhodnius prolixus, and a short-lived globally
invasive polyphagous agricultural pest, western flower thrips (Frankliniella
occidentalis). When ingested, the manipulated bacteria colonized the insects,
successfully competed with the wild-type microflora, and sustainably
mediated systemic knockdown phenotypes that were horizontally transmissible.
This represents a significant advance in the ability to deliver RNAi,
potentially to a large range of non-model insects.
KW - Biocide
KW - Chagas disease
KW - Insect
KW - RNA interference
KW - Symbiotic bacteria
UR - http://www.scopus.com/inward/record.url?scp=84959449966&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84959449966&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/f78c8d82-1c86-3a8c-9925-b0b6074f01f4/
U2 - 10.1098/rspb.2016.0042
DO - 10.1098/rspb.2016.0042
M3 - Article (journal)
C2 - 26911963
SN - 0962-8452
VL - 283
SP - 20160042
JO - Proceedings of the Royal Society B: Biological Sciences
JF - Proceedings of the Royal Society B: Biological Sciences
IS - 1825
M1 - 20160042
ER -
