TY - JOUR
T1 - Streptomyces altiplanensis sp. Nov., an alkalitolerant species isolated from chilean altiplano soil, and emended description of streptomyces chryseus (krasil’nikov et al. 1965) pridham 1970
AU - Cortes-Albayay, Carlos
AU - Dorador, Cristina
AU - Schumann, Peter
AU - Schniete, Jana K.
AU - Herron, Paul
AU - Andrews, Barbara
AU - Asenjo, Juan
AU - Nouioui, Imen
N1 - Funding Information:
Genome sequencing was provided by MicrobesNG (www. microbesng.uk), which is supported by the BBSRC (grant number BB/L024209/1). This project was supported by the School of Natural and Environmental Sciences at Newcastle University. I.N. is grateful to Newcastle University for a post-doctoral fellowship. C.CA. is grateful to CONICYT for a PFCHA/DOCTORADO BECAS CHILE/ 2016–21160585 fellowship and operational expenses. This work was also financially supported by the Basal Centres Programme of CONICYT (Chile) for funding the Centre for Biotechnology and Bioengineering, CeBiB (project FB0001) and FONDECYT 1110953; 11181773 grants.
Publisher Copyright:
© 2019 IUMS.
Copyright:
Copyright 2019 Elsevier B.V., All rights reserved.
PY - 2019/8/1
Y1 - 2019/8/1
N2 - A polyphasic approach was used for evaluating the taxonomic status of strain HST21T isolated from Salar de Huasco in the Atacama Desert. The results of 16S rRNA gene and multilocus sequence phylogenetic analyses assigned strain HST21T to the genus Streptomyces with Streptomyces albidochromogenes DSM 41800T and Streptomyces flavidovirens DSM 40150T as its nearest neighbours. Digital DNA–DNA hydridization (dDDH) and average nucleotide identity (ANI) values between the genome sequences of strain HST21T and S. albidochromogenes DSM 41800T (35.6 and 88.2 %) and S. flavidovirens DSM 40105T (47.2 and 88.8 %) were below the thresholds of 70 and 95–96 % for prokaryotic conspecific assignation. Phenotypic, chemotaxonomic and genetic results distinguished strain HST21T from its closest neighbours. Strain HST21T is characterized by the presence of LL-diaminopimelic acid in its peptidoglycan layer; glucose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, glycophospholipids, unknown lipids and phospholipids as polar lipids; and anteiso-C15: 0 (21.6 %) and anteiso-C17: 0 (20.5 %) as major fatty acids (>15 %). Based on these results, strain HST21T merits recognition as a novel species, for which the name Streptomyces altiplanensis sp. nov. is proposed. The type strain is HST21T =DSM 107267T =CECT 9647T . While analysing the phylogenies of strain HST21T, Streptomyces chryseus DSM 40420T and Streptomyces helvaticus DSM 40431T were found to have 100 % 16S rRNA gene sequence similarity with digital DNA–DNA hydridization (dDDH) and average nucleotide identity (ANI) values of 95.3 and 99.4 %, respectively. Therefore, S. helvaticus is considered as a later heterotypic synonym of S. chryseus and, consequently, we emend the description of S. chryseus.
AB - A polyphasic approach was used for evaluating the taxonomic status of strain HST21T isolated from Salar de Huasco in the Atacama Desert. The results of 16S rRNA gene and multilocus sequence phylogenetic analyses assigned strain HST21T to the genus Streptomyces with Streptomyces albidochromogenes DSM 41800T and Streptomyces flavidovirens DSM 40150T as its nearest neighbours. Digital DNA–DNA hydridization (dDDH) and average nucleotide identity (ANI) values between the genome sequences of strain HST21T and S. albidochromogenes DSM 41800T (35.6 and 88.2 %) and S. flavidovirens DSM 40105T (47.2 and 88.8 %) were below the thresholds of 70 and 95–96 % for prokaryotic conspecific assignation. Phenotypic, chemotaxonomic and genetic results distinguished strain HST21T from its closest neighbours. Strain HST21T is characterized by the presence of LL-diaminopimelic acid in its peptidoglycan layer; glucose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, glycophospholipids, unknown lipids and phospholipids as polar lipids; and anteiso-C15: 0 (21.6 %) and anteiso-C17: 0 (20.5 %) as major fatty acids (>15 %). Based on these results, strain HST21T merits recognition as a novel species, for which the name Streptomyces altiplanensis sp. nov. is proposed. The type strain is HST21T =DSM 107267T =CECT 9647T . While analysing the phylogenies of strain HST21T, Streptomyces chryseus DSM 40420T and Streptomyces helvaticus DSM 40431T were found to have 100 % 16S rRNA gene sequence similarity with digital DNA–DNA hydridization (dDDH) and average nucleotide identity (ANI) values of 95.3 and 99.4 %, respectively. Therefore, S. helvaticus is considered as a later heterotypic synonym of S. chryseus and, consequently, we emend the description of S. chryseus.
KW - Actinobacteria
KW - Atacama Desert
KW - Genome sequence
KW - Taxonomy
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U2 - 10.1099/ijsem.0.003525
DO - 10.1099/ijsem.0.003525
M3 - Article (journal)
C2 - 31204968
AN - SCOPUS:85071069188
SN - 1466-5026
VL - 69
SP - 2498
EP - 2505
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 8
M1 - 003525
ER -