Streptomyces altiplanensis sp. Nov., an alkalitolerant species isolated from chilean altiplano soil, and emended description of streptomyces chryseus (krasil’nikov et al. 1965) pridham 1970

A polyphasic approach was used for evaluating the taxonomic status of strain HST21^T isolated from Salar de Huasco in the Atacama Desert. The results of 16S rRNA gene and multilocus sequence phylogenetic analyses assigned strain HST21^T to the genus Streptomyces with Streptomyces albidochromogenes DSM 41800^T and Streptomyces flavidovirens DSM 40150^T as its nearest neighbours. Digital DNA–DNA hydridization (dDDH) and average nucleotide identity (ANI) values between the genome sequences of strain HST21^T and S. albidochromogenes DSM 41800^T (35.6 and 88.2 %) and S. flavidovirens DSM 40105^T (47.2 and 88.8 %) were below the thresholds of 70 and 95–96 % for prokaryotic conspecific assignation. Phenotypic, chemotaxonomic and genetic results distinguished strain HST21^T from its closest neighbours. Strain HST21^T is characterized by the presence of LL-diaminopimelic acid in its peptidoglycan layer; glucose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, glycophospholipids, unknown lipids and phospholipids as polar lipids; and anteiso-C_{15: 0} (21.6 %) and anteiso-C_{17: 0} (20.5 %) as major fatty acids (>15 %). Based on these results, strain HST21^T merits recognition as a novel species, for which the name Streptomyces altiplanensis sp. nov. is proposed. The type strain is HST21^T =DSM 107267^T =CECT 9647^T . While analysing the phylogenies of strain HST21^T, Streptomyces chryseus DSM 40420^T and Streptomyces helvaticus DSM 40431^T were found to have 100 % 16S rRNA gene sequence similarity with digital DNA–DNA hydridization (dDDH) and average nucleotide identity (ANI) values of 95.3 and 99.4 %, respectively. Therefore, S. helvaticus is considered as a later heterotypic synonym of S. chryseus and, consequently, we emend the description of S. chryseus.