Squamous cell carcinoma of the head and neck outside the oropharynx is rarely human papillomavirus related

Navdeep S Upile; Richard J Shaw; Terry M Jones; Paul Goodyear; Triantafillos Liloglou; Janet M Risk; Mark T Boyd; Jon Sheard; Phil Sloan; Max Robinson; Andrew G Schache

doi:10.1002/lary.24828

Squamous cell carcinoma of the head and neck outside the oropharynx is rarely human papillomavirus related

Navdeep S Upile
, Richard J Shaw
, Terry M Jones
, Paul Goodyear
, Triantafillos Liloglou
, Janet M Risk
, Mark T Boyd
, Jon Sheard
, Phil Sloan
, Max Robinson
, Andrew G Schache

Medical School

Research output: Contribution to journal › Article (journal) › peer-review

54 Citations (Scopus)

Abstract

OBJECTIVES/HYPOTHESIS: The incidence of human papillomavirus (HPV)-driven disease beyond the oropharynx varies greatly in the reported literature.

STUDY DESIGN: Case series.

METHODS: Two hundred twenty-one samples were strictly classified to the subsites of oral cavity, larynx, or hypopharynx at the time of primary surgery. Formalin-fixed paraffin-embedded samples were subjected to a validated, tiered, diagnostic algorithm of p16 immunohistochemistry, high-risk HPV in situ hybridization, and quantitative polymerase chain reaction for HPV E6 DNA. An additional 60 oropharyngeal cases acted as an internal biological control.

RESULTS: An incidence of 4% of HPV-driven cases was observed across the subsites outside the oropharynx compared to 70% of tumors confined within it.

CONCLUSIONS: This is the first reporting of a broad range of nonoropharyngeal HPV rates using this validated diagnostic algorithm. It remains unclear whether patients with HPV-driven disease originating outside the oropharynx enjoy the same survival advantage apparent in those patients with oropharyngeal squamous cell carcinomas.

LEVEL OF EVIDENCE: 4 Laryngoscope, 124:2739-2744, 2014.

Original language	English
Pages (from-to)	2739-44
Number of pages	6
Journal	Laryngoscope
Volume	124
Issue number	12
DOIs	https://doi.org/10.1002/lary.24828
Publication status	Published - 1 Dec 2014

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Biomarkers, Tumor/analysis
Carcinoma, Squamous Cell/diagnosis
DNA, Viral/analysis
Diagnosis, Differential
Female
Follow-Up Studies
Head and Neck Neoplasms/diagnosis
Humans
Immunohistochemistry
In Situ Hybridization
Male
Middle Aged
Oropharynx
Papillomaviridae/genetics
Papillomavirus Infections/diagnosis
Polymerase Chain Reaction
Real-Time Polymerase Chain Reaction
Retrospective Studies
Squamous Cell Carcinoma of Head and Neck

Access to Document

10.1002/lary.24828

Cite this

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title = "Squamous cell carcinoma of the head and neck outside the oropharynx is rarely human papillomavirus related",

abstract = "OBJECTIVES/HYPOTHESIS: The incidence of human papillomavirus (HPV)-driven disease beyond the oropharynx varies greatly in the reported literature.STUDY DESIGN: Case series.METHODS: Two hundred twenty-one samples were strictly classified to the subsites of oral cavity, larynx, or hypopharynx at the time of primary surgery. Formalin-fixed paraffin-embedded samples were subjected to a validated, tiered, diagnostic algorithm of p16 immunohistochemistry, high-risk HPV in situ hybridization, and quantitative polymerase chain reaction for HPV E6 DNA. An additional 60 oropharyngeal cases acted as an internal biological control.RESULTS: An incidence of 4\% of HPV-driven cases was observed across the subsites outside the oropharynx compared to 70\% of tumors confined within it.CONCLUSIONS: This is the first reporting of a broad range of nonoropharyngeal HPV rates using this validated diagnostic algorithm. It remains unclear whether patients with HPV-driven disease originating outside the oropharynx enjoy the same survival advantage apparent in those patients with oropharyngeal squamous cell carcinomas.LEVEL OF EVIDENCE: 4 Laryngoscope, 124:2739-2744, 2014.",

keywords = "Biomarkers, Tumor/analysis, Carcinoma, Squamous Cell/diagnosis, DNA, Viral/analysis, Diagnosis, Differential, Female, Follow-Up Studies, Head and Neck Neoplasms/diagnosis, Humans, Immunohistochemistry, In Situ Hybridization, Male, Middle Aged, Oropharynx, Papillomaviridae/genetics, Papillomavirus Infections/diagnosis, Polymerase Chain Reaction, Real-Time Polymerase Chain Reaction, Retrospective Studies, Squamous Cell Carcinoma of Head and Neck",

author = "Upile, \{Navdeep S\} and Shaw, \{Richard J\} and Jones, \{Terry M\} and Paul Goodyear and Triantafillos Liloglou and Risk, \{Janet M\} and Boyd, \{Mark T\} and Jon Sheard and Phil Sloan and Max Robinson and Schache, \{Andrew G\}",

note = "{\textcopyright} 2014 The American Laryngological, Rhinological and Otological Society, Inc.",

year = "2014",

month = dec,

day = "1",

doi = "10.1002/lary.24828",

language = "English",

volume = "124",

pages = "2739--44",

journal = "Laryngoscope",

issn = "0023-852X",

publisher = "Wiley-Blackwell",

number = "12",

}

TY - JOUR

T1 - Squamous cell carcinoma of the head and neck outside the oropharynx is rarely human papillomavirus related

AU - Upile, Navdeep S

AU - Shaw, Richard J

AU - Jones, Terry M

AU - Goodyear, Paul

AU - Liloglou, Triantafillos

AU - Risk, Janet M

AU - Boyd, Mark T

AU - Sheard, Jon

AU - Sloan, Phil

AU - Robinson, Max

AU - Schache, Andrew G

PY - 2014/12/1

Y1 - 2014/12/1

N2 - OBJECTIVES/HYPOTHESIS: The incidence of human papillomavirus (HPV)-driven disease beyond the oropharynx varies greatly in the reported literature.STUDY DESIGN: Case series.METHODS: Two hundred twenty-one samples were strictly classified to the subsites of oral cavity, larynx, or hypopharynx at the time of primary surgery. Formalin-fixed paraffin-embedded samples were subjected to a validated, tiered, diagnostic algorithm of p16 immunohistochemistry, high-risk HPV in situ hybridization, and quantitative polymerase chain reaction for HPV E6 DNA. An additional 60 oropharyngeal cases acted as an internal biological control.RESULTS: An incidence of 4% of HPV-driven cases was observed across the subsites outside the oropharynx compared to 70% of tumors confined within it.CONCLUSIONS: This is the first reporting of a broad range of nonoropharyngeal HPV rates using this validated diagnostic algorithm. It remains unclear whether patients with HPV-driven disease originating outside the oropharynx enjoy the same survival advantage apparent in those patients with oropharyngeal squamous cell carcinomas.LEVEL OF EVIDENCE: 4 Laryngoscope, 124:2739-2744, 2014.

AB - OBJECTIVES/HYPOTHESIS: The incidence of human papillomavirus (HPV)-driven disease beyond the oropharynx varies greatly in the reported literature.STUDY DESIGN: Case series.METHODS: Two hundred twenty-one samples were strictly classified to the subsites of oral cavity, larynx, or hypopharynx at the time of primary surgery. Formalin-fixed paraffin-embedded samples were subjected to a validated, tiered, diagnostic algorithm of p16 immunohistochemistry, high-risk HPV in situ hybridization, and quantitative polymerase chain reaction for HPV E6 DNA. An additional 60 oropharyngeal cases acted as an internal biological control.RESULTS: An incidence of 4% of HPV-driven cases was observed across the subsites outside the oropharynx compared to 70% of tumors confined within it.CONCLUSIONS: This is the first reporting of a broad range of nonoropharyngeal HPV rates using this validated diagnostic algorithm. It remains unclear whether patients with HPV-driven disease originating outside the oropharynx enjoy the same survival advantage apparent in those patients with oropharyngeal squamous cell carcinomas.LEVEL OF EVIDENCE: 4 Laryngoscope, 124:2739-2744, 2014.

KW - Biomarkers, Tumor/analysis

KW - Carcinoma, Squamous Cell/diagnosis

KW - DNA, Viral/analysis

KW - Diagnosis, Differential

KW - Female

KW - Follow-Up Studies

KW - Head and Neck Neoplasms/diagnosis

KW - Humans

KW - Immunohistochemistry

KW - In Situ Hybridization

KW - Male

KW - Middle Aged

KW - Oropharynx

KW - Papillomaviridae/genetics

KW - Papillomavirus Infections/diagnosis

KW - Polymerase Chain Reaction

KW - Real-Time Polymerase Chain Reaction

KW - Retrospective Studies

KW - Squamous Cell Carcinoma of Head and Neck

U2 - 10.1002/lary.24828

DO - 10.1002/lary.24828

M3 - Article (journal)

C2 - 25042603

SN - 0023-852X

VL - 124

SP - 2739

EP - 2744

JO - Laryngoscope

JF - Laryngoscope

IS - 12

ER -

Squamous cell carcinoma of the head and neck outside the oropharynx is rarely human papillomavirus related

Abstract

UN SDGs

Keywords

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