Simultaneous monitoring of vascular contractility, intracellular pH and intracellular calcium in isolated rat mesenteric arteries; effects of weak bases

D Otter, C Austin

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We report the first simultaneous measurements of pHi, [Ca2+]i and tension, upon alteration of pHi, in isolated rat mesenteric arteries loaded with both carboxy SNARF and indo-1. In these vessels (pre-contracted by 30 mM KCl) alterations of pHi, by addition and subsequent washout of weak bases, produced complicated effects on tone. Although the changes in contractility did not mirror the changes in pHi they were, at all times, accompanied by parallel changes in [Ca2+]i.

Original languageEnglish
Pages (from-to)349-51
Number of pages3
JournalExperimental Physiology
Volume85
Issue number3
Publication statusPublished - 31 May 2000

Keywords

  • Alkalies
  • Ammonium Chloride/pharmacology
  • Animals
  • Benzopyrans
  • Calcium/chemistry
  • Fluorescent Dyes
  • Hydrogen-Ion Concentration
  • Indoles
  • Male
  • Mesenteric Arteries/chemistry
  • Methylamines/pharmacology
  • Muscle Contraction/physiology
  • Muscle, Smooth, Vascular/chemistry
  • Naphthols
  • Potassium Chloride/pharmacology
  • Rats
  • Rats, Wistar
  • Rhodamines

Fingerprint Dive into the research topics of 'Simultaneous monitoring of vascular contractility, intracellular pH and intracellular calcium in isolated rat mesenteric arteries; effects of weak bases'. Together they form a unique fingerprint.

  • Cite this