Simultaneous measurement of intracellular pH, calcium, and tension in rat mesenteric vessels: effects of extracellular pH

K Dilly, D Eisner, S Wray, CLARE AUSTIN

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

In rat mesenteric vessels changes in external pH (pHo) alter tension. Changes in intracellular pH (pHi) and calcium ([Ca2+]i) have both been suggested to underlie the tension changes. As no simultaneous measurements of pHi and [Ca2+]i had been made, however, the relative importance and temporal relationship between pHi [Ca2+]i and tension were unknown. In order therefore to gain a clearer understanding of the mechanisms involved, we have made simultaneous measurements of these parameters using carboxy-SNARF and INDO-1. Raising pHo caused rises in pHi, Ca2+ and tension. The increases in pHi preceded increases in [Ca2+]i, which preceded the increases in tension. Similar but opposite effects were observed when pHo was decreased. We conclude that the change in [Ca2+]i upon alteration of pHo is subsequent to that of pHi.

Original languageEnglish
Pages (from-to)537-40
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume222
Issue number2
DOIs
Publication statusPublished - 15 May 1996

Keywords

  • Animals
  • Calcium/metabolism
  • Fluorescent Dyes
  • Hydrogen-Ion Concentration
  • In Vitro Techniques
  • Indoles
  • Male
  • Muscle, Smooth, Vascular/physiology
  • Rats
  • Rats, Wistar
  • Spectrometry, Fluorescence
  • Splanchnic Circulation
  • Stress, Mechanical

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