Primary porcine brain microvessel endothelial cell isolation and culture.

A Patabendige*, NJ Abbott

*Corresponding author for this work

Research output: Contribution to journalArticle (journal)peer-review

14 Citations (Scopus)

Abstract

Cell culture models of the blood-brain barrier (BBB) are useful tools to study the functionality of the BBB in health and disease. Several good in vitro BBB models are available from different species. However, most brain endothelial cells lose some of their in vivo BBB phenotype in culture. Porcine brain endothelial cells (PBECs) tend to retain most of their in vivo BBB characteristics and usually give higher transendothelial electrical resistance (TEER, representing functional well-developed tight junctions) compared to brain endothelial cells from other species. The protocol described in this unit gives detailed instructions for isolation and culture of PBECs from fresh porcine brains. This porcine BBB model generates high TEER without the need for co-culture with astrocytes. However, astrocyte-derived factors can be introduced to the system through the use of astrocyte-conditioned medium or co-culture with astrocytes, which may be necessary for further enhancing the BBB phenotype for certain complex studies. Curr.
Original languageEnglish
Article number25297692
Pages (from-to)3.27.1-3.27.17
JournalCurrent Protocols in Neuroscience
Volume69
DOIs
Publication statusPublished - 8 Oct 2014

Keywords

  • Astrocyte-conditioned medium
  • Blood-brain barrier
  • Cell culture
  • Porcine brain endothelial cells
  • Transendothelial electrical resistance

Fingerprint

Dive into the research topics of 'Primary porcine brain microvessel endothelial cell isolation and culture.'. Together they form a unique fingerprint.

Cite this