Preliminary investigation of the prevalence and implantation potential of abnormal embryonic phenotypes assessed using time-lapse imaging

Amy Barrie, Roy Homburg, Gary McDowell, Jeremy Brown, Charles Kingsland, Stephen Troup

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

This retrospective, single site observational study aimed to delineate five abnormal embryonic developmental phenotypes, assessing their prevalence, development potential and suitability for inclusion in embryo selection models for IVF. In total, 15,819 embryos from 4559 treatment cycles cultured in EmbryoScope® incubators between January 2014 and January 2016 were included. Time-lapse images were assessed retrospectively for five abnormal embryo phenotypes: direct cleavage, reverse cleavage, absent cleavage, chaotic cleavage and cell lysis. The prevalence of each abnormal phenotype was assessed. Final embryo disposition, embryo quality and implantation rate were determined and compared with a control embryo cohort. The collective prevalence for the five abnormal phenotypes was 11.4%; chaotic cleavage and direct cleavage together constituted 9.7%. Implantation rates were 17.4%, 0%, 25%, 2.1% and 0% for direct, reverse, absent, chaotic cleavage and cell lysis, respectively. The overall implantation rate for all abnormal embryos with known implantation status was significantly lower compared with the control population (6.9% versus 38.7%, P < 0.0001). The proportion of good quality embryos in each category of abnormal cleavage remained below 25%. Embryos exhibiting an abnormal phenotype may have reduced developmental capability, manifested in both embryo quality and implantation potential, when compared with embryos of normal phenotype.
Original languageEnglish
JournalReproductive BioMedicine Online
Early online date1 Mar 2017
DOIs
Publication statusE-pub ahead of print - 1 Mar 2017

Fingerprint

Phenotype
Embryonic Structures
Embryo Disposition
Incubators
Population

Keywords

  • Abnormal phenotype
  • Embryodevelopment
  • Morphokinetics
  • Time-lapse

Cite this

@article{74b5ebd3fa7847e9a28d3c4bddcf3f8f,
title = "Preliminary investigation of the prevalence and implantation potential of abnormal embryonic phenotypes assessed using time-lapse imaging",
abstract = "This retrospective, single site observational study aimed to delineate five abnormal embryonic developmental phenotypes, assessing their prevalence, development potential and suitability for inclusion in embryo selection models for IVF. In total, 15,819 embryos from 4559 treatment cycles cultured in EmbryoScope{\circledR} incubators between January 2014 and January 2016 were included. Time-lapse images were assessed retrospectively for five abnormal embryo phenotypes: direct cleavage, reverse cleavage, absent cleavage, chaotic cleavage and cell lysis. The prevalence of each abnormal phenotype was assessed. Final embryo disposition, embryo quality and implantation rate were determined and compared with a control embryo cohort. The collective prevalence for the five abnormal phenotypes was 11.4{\%}; chaotic cleavage and direct cleavage together constituted 9.7{\%}. Implantation rates were 17.4{\%}, 0{\%}, 25{\%}, 2.1{\%} and 0{\%} for direct, reverse, absent, chaotic cleavage and cell lysis, respectively. The overall implantation rate for all abnormal embryos with known implantation status was significantly lower compared with the control population (6.9{\%} versus 38.7{\%}, P < 0.0001). The proportion of good quality embryos in each category of abnormal cleavage remained below 25{\%}. Embryos exhibiting an abnormal phenotype may have reduced developmental capability, manifested in both embryo quality and implantation potential, when compared with embryos of normal phenotype.",
keywords = "Abnormal phenotype, Embryodevelopment, Morphokinetics, Time-lapse",
author = "Amy Barrie and Roy Homburg and Gary McDowell and Jeremy Brown and Charles Kingsland and Stephen Troup",
year = "2017",
month = "3",
day = "1",
doi = "10.1016/j.rbmo.2017.02.011",
language = "English",
journal = "Reproductive BioMedicine Online",
issn = "1472-6483",
publisher = "Elsevier",

}

Preliminary investigation of the prevalence and implantation potential of abnormal embryonic phenotypes assessed using time-lapse imaging. / Barrie, Amy; Homburg, Roy; McDowell, Gary; Brown, Jeremy; Kingsland, Charles; Troup, Stephen.

In: Reproductive BioMedicine Online, 01.03.2017.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Preliminary investigation of the prevalence and implantation potential of abnormal embryonic phenotypes assessed using time-lapse imaging

AU - Barrie, Amy

AU - Homburg, Roy

AU - McDowell, Gary

AU - Brown, Jeremy

AU - Kingsland, Charles

AU - Troup, Stephen

PY - 2017/3/1

Y1 - 2017/3/1

N2 - This retrospective, single site observational study aimed to delineate five abnormal embryonic developmental phenotypes, assessing their prevalence, development potential and suitability for inclusion in embryo selection models for IVF. In total, 15,819 embryos from 4559 treatment cycles cultured in EmbryoScope® incubators between January 2014 and January 2016 were included. Time-lapse images were assessed retrospectively for five abnormal embryo phenotypes: direct cleavage, reverse cleavage, absent cleavage, chaotic cleavage and cell lysis. The prevalence of each abnormal phenotype was assessed. Final embryo disposition, embryo quality and implantation rate were determined and compared with a control embryo cohort. The collective prevalence for the five abnormal phenotypes was 11.4%; chaotic cleavage and direct cleavage together constituted 9.7%. Implantation rates were 17.4%, 0%, 25%, 2.1% and 0% for direct, reverse, absent, chaotic cleavage and cell lysis, respectively. The overall implantation rate for all abnormal embryos with known implantation status was significantly lower compared with the control population (6.9% versus 38.7%, P < 0.0001). The proportion of good quality embryos in each category of abnormal cleavage remained below 25%. Embryos exhibiting an abnormal phenotype may have reduced developmental capability, manifested in both embryo quality and implantation potential, when compared with embryos of normal phenotype.

AB - This retrospective, single site observational study aimed to delineate five abnormal embryonic developmental phenotypes, assessing their prevalence, development potential and suitability for inclusion in embryo selection models for IVF. In total, 15,819 embryos from 4559 treatment cycles cultured in EmbryoScope® incubators between January 2014 and January 2016 were included. Time-lapse images were assessed retrospectively for five abnormal embryo phenotypes: direct cleavage, reverse cleavage, absent cleavage, chaotic cleavage and cell lysis. The prevalence of each abnormal phenotype was assessed. Final embryo disposition, embryo quality and implantation rate were determined and compared with a control embryo cohort. The collective prevalence for the five abnormal phenotypes was 11.4%; chaotic cleavage and direct cleavage together constituted 9.7%. Implantation rates were 17.4%, 0%, 25%, 2.1% and 0% for direct, reverse, absent, chaotic cleavage and cell lysis, respectively. The overall implantation rate for all abnormal embryos with known implantation status was significantly lower compared with the control population (6.9% versus 38.7%, P < 0.0001). The proportion of good quality embryos in each category of abnormal cleavage remained below 25%. Embryos exhibiting an abnormal phenotype may have reduced developmental capability, manifested in both embryo quality and implantation potential, when compared with embryos of normal phenotype.

KW - Abnormal phenotype

KW - Embryodevelopment

KW - Morphokinetics

KW - Time-lapse

U2 - 10.1016/j.rbmo.2017.02.011

DO - 10.1016/j.rbmo.2017.02.011

M3 - Article

JO - Reproductive BioMedicine Online

JF - Reproductive BioMedicine Online

SN - 1472-6483

ER -