Abstract
Cystatin C is a cysteine protease inhibitor expressed by the retinal pigment epithelium (RPE). The previously characterised variant B version of this protein has been associated with an increased risk of developing exudative age-related macular degeneration (AMD). The aim of this study was to optimize the culture and manipulation of human fetal RPE (hfRPE) monolayers, in order to analyze the secretion of the endogenous cystatin C, as well as eGFP fused wild-type and variant B cystatin C constructs.
Original language | English |
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Pages (from-to) | e1604 |
Journal | Investigative Ophthalmology and Visual Science |
Volume | 53 |
Issue number | 14 |
Publication status | Published - 1 Mar 2012 |