Pharmacological effects of asiatic acid in glioblastoma cells under hypoxia

Flourina Kumar Thakor, Ka-Wai Wan, Philip John Welsby, Gail Welsby

Research output: Contribution to journalArticle

7 Citations (Scopus)
11 Downloads (Pure)

Abstract

Glioblastoma multiforme is the most common and malignant primary brain tumor in adults. Despite current treatment options including surgery followed by radiation and chemotherapy with temozolomide and cisplatin, the median survival rate remains low (<16 months). Combined with increasing drug resistance and the inability of some compounds to cross the blood-brain barrier, novel compounds are being sought for the treatment of this disease. Here, we aimed to examine the pharmacological effect of Asiatic acid (AA) in glioblastoma under hypoxia. To investigate the effects of AA on cell viability, proliferation, apoptosis, and wound healing, SVG p12 fetal glia and U87-MG grade IV glioblastoma cells were cultured under normoxic (21% O2) and hypoxic (1% O2) conditions. In normoxia, AA reduced cell viability in U87-MG cells in a time and concentration-dependent manner. A significant decrease in viability, compared to cisplatin, was observed following 2 h of AA treatment with no significant changes in cell proliferation or cell cycle progression observed. Under hypoxia, a significantly greater number of cells underwent apoptosis in comparison to cisplatin. While cisplatin showed a reduction in wound healing in normoxia, a significantly greater reduction was observed following AA treatment. An overall reduction in wound healing was observed under hypoxia. The results of this study show that AA has cytotoxic effects on glioma cell lines and has the potential to become an alternative treatment for glioblastoma.

Original languageEnglish
Pages (from-to)179-190
Number of pages12
JournalMolecular and Cellular Biochemistry
Volume430
Issue number1-2
Early online date15 Feb 2017
DOIs
Publication statusPublished - 30 Jun 2017

Fingerprint

Cell Hypoxia
Glioblastoma
Pharmacology
Cisplatin
Wound Healing
temozolomide
Cells
Cell proliferation
Cell Survival
Cell Proliferation
Apoptosis
Therapeutics
Chemotherapy
Blood-Brain Barrier
Drug Resistance
Brain Neoplasms
Glioma
Neuroglia
Surgery
asiatic acid

Keywords

  • Cell Cycle/drug effects
  • Cell Hypoxia/drug effects
  • Cell Line, Tumor
  • Cell Survival/drug effects
  • Glioblastoma/drug therapy
  • Humans
  • Pentacyclic Triterpenes/pharmacology

Cite this

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abstract = "Glioblastoma multiforme is the most common and malignant primary brain tumor in adults. Despite current treatment options including surgery followed by radiation and chemotherapy with temozolomide and cisplatin, the median survival rate remains low (<16 months). Combined with increasing drug resistance and the inability of some compounds to cross the blood-brain barrier, novel compounds are being sought for the treatment of this disease. Here, we aimed to examine the pharmacological effect of Asiatic acid (AA) in glioblastoma under hypoxia. To investigate the effects of AA on cell viability, proliferation, apoptosis, and wound healing, SVG p12 fetal glia and U87-MG grade IV glioblastoma cells were cultured under normoxic (21{\%} O2) and hypoxic (1{\%} O2) conditions. In normoxia, AA reduced cell viability in U87-MG cells in a time and concentration-dependent manner. A significant decrease in viability, compared to cisplatin, was observed following 2 h of AA treatment with no significant changes in cell proliferation or cell cycle progression observed. Under hypoxia, a significantly greater number of cells underwent apoptosis in comparison to cisplatin. While cisplatin showed a reduction in wound healing in normoxia, a significantly greater reduction was observed following AA treatment. An overall reduction in wound healing was observed under hypoxia. The results of this study show that AA has cytotoxic effects on glioma cell lines and has the potential to become an alternative treatment for glioblastoma.",
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Pharmacological effects of asiatic acid in glioblastoma cells under hypoxia. / Thakor, Flourina Kumar; Wan, Ka-Wai; Welsby, Philip John; Welsby, Gail.

In: Molecular and Cellular Biochemistry, Vol. 430, No. 1-2, 30.06.2017, p. 179-190.

Research output: Contribution to journalArticle

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