p53 mutations in relation to human papillomavirus type 16 infection in squamous cell carcinomas of the head and neck

A G Scholes; T Liloglou; P J Snijders; C A Hart; A S Jones; J A Woolgar; E D Vaughan; J M Walboomers; J K Field

doi:10.1002/(sici)1097-0215(19970529)71:5<796::aid-ijc17>3.0.co;2-6

p53 mutations in relation to human papillomavirus type 16 infection in squamous cell carcinomas of the head and neck

A G Scholes, T Liloglou, P J Snijders, C A Hart, A S Jones, J A Woolgar, E D Vaughan, J M Walboomers, J K Field

University of Liverpool

Research output: Contribution to journal › Article (journal) › peer-review

28 Citations (Scopus)

Abstract

The relationship between human papillomavirus (HPV) type 16 infection and p53 gene mutations was investigated in squamous cell carcinomas of the head and neck (SCCHN). HPV was detected by general primer-mediated and type-specific PCR. Alterations in the p53 gene were investigated using single-strand conformation polymorphism and sequence analysis in 27 SCCHN, of which 12 were HPV 16-positive and 15 were HPV-negative. Mutations were detected in 2/12 (16.7%) HPV 16-positive and 7/15 (46.7%) HPV-negative tumours; this difference was not statistically significant. The predominant mutations were deletions and C --> T transitions; G --> T transversions were found in only 2 tumours. Our results indicate that the presence of HPV 16 and p53 mutations is not mutually exclusive and detection of a p53 mutation does not exclude a potential role for HPV 16 in the pathogenesis of a subset of SCCHN.

Original language	English
Pages (from-to)	796-9
Number of pages	4
Journal	International Journal of Cancer
Volume	71
Issue number	5
DOIs	https://doi.org/10.1002/(sici)1097-0215(19970529)71:5<796::aid-ijc17>3.0.co;2-6
Publication status	Published - 6 Dec 1998

Keywords

Carcinoma, Squamous Cell/genetics
Exons
Gene Deletion
Genes, p53/genetics
Head and Neck Neoplasms/genetics
Humans
Mutation
Papillomaviridae/isolation & purification
Papillomavirus Infections/genetics
Polymerase Chain Reaction
Polymorphism, Single-Stranded Conformational
Smoking
Tumor Virus Infections/genetics

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10.1002/(sici)1097-0215(19970529)71:5<796::aid-ijc17>3.0.co;2-6

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Scholes, A. G., Liloglou, T., Snijders, P. J., Hart, C. A., Jones, A. S., Woolgar, J. A., Vaughan, E. D., Walboomers, J. M., & Field, J. K. (1998). p53 mutations in relation to human papillomavirus type 16 infection in squamous cell carcinomas of the head and neck. International Journal of Cancer, 71(5), 796-9. https://doi.org/10.1002/(sici)1097-0215(19970529)71:5<796::aid-ijc17>3.0.co;2-6

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abstract = "The relationship between human papillomavirus (HPV) type 16 infection and p53 gene mutations was investigated in squamous cell carcinomas of the head and neck (SCCHN). HPV was detected by general primer-mediated and type-specific PCR. Alterations in the p53 gene were investigated using single-strand conformation polymorphism and sequence analysis in 27 SCCHN, of which 12 were HPV 16-positive and 15 were HPV-negative. Mutations were detected in 2/12 (16.7%) HPV 16-positive and 7/15 (46.7%) HPV-negative tumours; this difference was not statistically significant. The predominant mutations were deletions and C --> T transitions; G --> T transversions were found in only 2 tumours. Our results indicate that the presence of HPV 16 and p53 mutations is not mutually exclusive and detection of a p53 mutation does not exclude a potential role for HPV 16 in the pathogenesis of a subset of SCCHN.",

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author = "Scholes, {A G} and T Liloglou and Snijders, {P J} and Hart, {C A} and Jones, {A S} and Woolgar, {J A} and Vaughan, {E D} and Walboomers, {J M} and Field, {J K}",

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Scholes, AG, Liloglou, T, Snijders, PJ, Hart, CA, Jones, AS, Woolgar, JA, Vaughan, ED, Walboomers, JM & Field, JK 1998, 'p53 mutations in relation to human papillomavirus type 16 infection in squamous cell carcinomas of the head and neck', International Journal of Cancer, vol. 71, no. 5, pp. 796-9. https://doi.org/10.1002/(sici)1097-0215(19970529)71:5<796::aid-ijc17>3.0.co;2-6

TY - JOUR

T1 - p53 mutations in relation to human papillomavirus type 16 infection in squamous cell carcinomas of the head and neck

AU - Scholes, A G

AU - Liloglou, T

AU - Snijders, P J

AU - Hart, C A

AU - Jones, A S

AU - Woolgar, J A

AU - Vaughan, E D

AU - Walboomers, J M

AU - Field, J K

PY - 1998/12/6

Y1 - 1998/12/6

N2 - The relationship between human papillomavirus (HPV) type 16 infection and p53 gene mutations was investigated in squamous cell carcinomas of the head and neck (SCCHN). HPV was detected by general primer-mediated and type-specific PCR. Alterations in the p53 gene were investigated using single-strand conformation polymorphism and sequence analysis in 27 SCCHN, of which 12 were HPV 16-positive and 15 were HPV-negative. Mutations were detected in 2/12 (16.7%) HPV 16-positive and 7/15 (46.7%) HPV-negative tumours; this difference was not statistically significant. The predominant mutations were deletions and C --> T transitions; G --> T transversions were found in only 2 tumours. Our results indicate that the presence of HPV 16 and p53 mutations is not mutually exclusive and detection of a p53 mutation does not exclude a potential role for HPV 16 in the pathogenesis of a subset of SCCHN.

AB - The relationship between human papillomavirus (HPV) type 16 infection and p53 gene mutations was investigated in squamous cell carcinomas of the head and neck (SCCHN). HPV was detected by general primer-mediated and type-specific PCR. Alterations in the p53 gene were investigated using single-strand conformation polymorphism and sequence analysis in 27 SCCHN, of which 12 were HPV 16-positive and 15 were HPV-negative. Mutations were detected in 2/12 (16.7%) HPV 16-positive and 7/15 (46.7%) HPV-negative tumours; this difference was not statistically significant. The predominant mutations were deletions and C --> T transitions; G --> T transversions were found in only 2 tumours. Our results indicate that the presence of HPV 16 and p53 mutations is not mutually exclusive and detection of a p53 mutation does not exclude a potential role for HPV 16 in the pathogenesis of a subset of SCCHN.

KW - Carcinoma, Squamous Cell/genetics

KW - Exons

KW - Gene Deletion

KW - Genes, p53/genetics

KW - Head and Neck Neoplasms/genetics

KW - Humans

KW - Mutation

KW - Papillomaviridae/isolation & purification

KW - Papillomavirus Infections/genetics

KW - Polymerase Chain Reaction

KW - Polymorphism, Single-Stranded Conformational

KW - Smoking

KW - Tumor Virus Infections/genetics

U2 - 10.1002/(sici)1097-0215(19970529)71:5<796::aid-ijc17>3.0.co;2-6

DO - 10.1002/(sici)1097-0215(19970529)71:5<796::aid-ijc17>3.0.co;2-6

M3 - Article (journal)

C2 - 9180148

SN - 0020-7136

VL - 71

SP - 796

EP - 799

JO - International Journal of Cancer

JF - International Journal of Cancer

IS - 5

ER -

p53 mutations in relation to human papillomavirus type 16 infection in squamous cell carcinomas of the head and neck

Abstract

Keywords

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