Microsatellite instability and loss of heterozygosity at the MEN1 locus in lung carcinoid tumors: a novel approach using real-time PCR with melting curve analysis in histopathologic material

Dimitra Vageli, Zoe Daniil, Jubrail Dahabreh, Eleni Karagianni, Triantafillos Liloglou, George Koukoulis, Konstantinos Gourgoulianis

Research output: Contribution to journalArticle (journal)peer-review

13 Citations (Scopus)

Abstract

The possible causes and genetic mechanisms of pulmonary carcinoid tumor development are unclear. In this study, we examined genetic alterations at the MEN1 locus in archival material from 15 pulmonary carcinoids. We employed, for the first time in this setting, real-time PCR with melting curve analysis in order to identify loss of heterozygosity (LOH) or microsatellite instability (MI) in two polymorphic markers (PYGM, D11S449) at the MEN1 locus and one additional marker (D11S906) of a putative oncosuppressive region distal to the MEN1 gene. Sequencing data were available in a selected subset of tumors in order to verify the reliability of real-time PCR analysis. We observed LOH at PYGM in 38% of the cases and MI in 13.3% of the cases. Our data indicate that real-time PCR with melting curve analysis is a reliable technique for LOH and MI detection and indicate that genetic errors at the MEN1 locus but also distal to it may be involved in the development of sporadic pulmonary carcinoid tumors.

Original languageEnglish
Pages (from-to)557-64
Number of pages8
JournalOncology Reports
Volume15
Issue number3
Publication statusPublished - 1 Mar 2006

Keywords

  • Adult
  • Aged
  • Base Sequence
  • Carcinoid Tumor/genetics
  • Child
  • DNA Mutational Analysis
  • DNA, Neoplasm/chemistry
  • Female
  • Humans
  • Loss of Heterozygosity
  • Lung Neoplasms/genetics
  • Male
  • Microsatellite Repeats/genetics
  • Middle Aged
  • Molecular Sequence Data
  • Polymerase Chain Reaction/methods
  • Proto-Oncogene Proteins/genetics
  • Time Factors
  • Transition Temperature

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