Metagenomic analysis of the bacterial microbiota associated with cultured oysters (Crassostrea sp.) in estuarine environments

Aline Horodesky*, Gisela G. Castilho-Westphal, Giorgi Dal Pont, Helisson Faoro, Eduardo Balsanelli, Michelle Z. Tadra-Sfeir, Nathieli Cozer, Marcio Roberto Pie, Antonio Ostrensky

*Corresponding author for this work

Research output: Contribution to journalArticle (journal)peer-review

4 Citations (Scopus)
5 Downloads (Pure)

Abstract

In this work, we identified the bacterial microbiota associated with farmed oystersin estuarine regions of four states in the north eastern region of Brazil. During the drought and rainy seasons, for eight months, twenty oysters were sampled seasonally from seven different marine farms. In the laboratory, DNA extraction, amplification, and sequencing of the 16S rRNA gene were performed to establish the taxonomic units. We identified 106 genera of bacteria belonging to 103 families, 70 orders, 39 classes, and 21 phyla. Out of the total, 40 of the genera represented bacteria potentially pathogenic to humans; of these, nine are known to cause foodborne diseases and six are potentially pathogenic to oysters. The most prevalent genera were Mycoplasma, Propionigenium, Psychrilyobacter, and Arcobacter. The results indicate the need for more systematic monitoring of bacteria of the genus Mycoplasma in oyster farming operations in the Brazilian north eastern region. Currently, Mycoplasma is not one of the microorganisms analysed and monitored by order of Brazilian legislation during the oyster production and/or commercialization process, even though this genus was the most prevalent at all sampling points and presents pathogenic potential both for oysters and for consumers.

Original languageEnglish
Article numbere20180432
Pages (from-to)1-15
Number of pages15
JournalAnais da Academia Brasileira de Ciencias
Volume92
Early online date26 Jun 2020
DOIs
Publication statusPublished - 26 Jun 2020

Keywords

  • Contamination
  • Cultivation
  • Oyster farming
  • Pathogenicity

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