TY - JOUR
T1 - Metagenomic analysis of the bacterial microbiota associated with cultured oysters (Crassostrea sp.) in estuarine environments
AU - Horodesky, Aline
AU - Castilho-Westphal, Gisela G.
AU - Pont, Giorgi Dal
AU - Faoro, Helisson
AU - Balsanelli, Eduardo
AU - Tadra-Sfeir, Michelle Z.
AU - Cozer, Nathieli
AU - Pie, Marcio Roberto
AU - Ostrensky, Antonio
N1 - Funding Information:
We thank the ?Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico? (CNPq) for support of this study by awarding the Productivity in Research Scholarship to Dr. Antonio Ostrensky (process 302609/2013-0) and by financing the project-associated processes #403381/2016-9. We also thank ?Servi?o Brasileiro de Apoio ?s Micro e Pequenas Empresas? for financial support to make this work possible.
Funding Information:
We thank the “Conselho Nacional de Desenvolvimento Científico e Tecnológico” (CNPq) for support of this study by awarding the Productivity in Research Scholarship to Dr. Antonio Ostrensky (process 302609/2013-0) and by financing the project-associated processes #403381/2016-9. We also thank “Serviço Brasileiro de Apoio às Micro e Pequenas Empresas” for financial support to make this work possible.
Publisher Copyright:
© 2020, Academia Brasileira de Ciencias. All rights reserved.
PY - 2020/6/26
Y1 - 2020/6/26
N2 - In this work, we identified the bacterial microbiota associated with farmed oystersin estuarine regions of four states in the north eastern region of Brazil. During the drought and rainy seasons, for eight months, twenty oysters were sampled seasonally from seven different marine farms. In the laboratory, DNA extraction, amplification, and sequencing of the 16S rRNA gene were performed to establish the taxonomic units. We identified 106 genera of bacteria belonging to 103 families, 70 orders, 39 classes, and 21 phyla. Out of the total, 40 of the genera represented bacteria potentially pathogenic to humans; of these, nine are known to cause foodborne diseases and six are potentially pathogenic to oysters. The most prevalent genera were Mycoplasma, Propionigenium, Psychrilyobacter, and Arcobacter. The results indicate the need for more systematic monitoring of bacteria of the genus Mycoplasma in oyster farming operations in the Brazilian north eastern region. Currently, Mycoplasma is not one of the microorganisms analysed and monitored by order of Brazilian legislation during the oyster production and/or commercialization process, even though this genus was the most prevalent at all sampling points and presents pathogenic potential both for oysters and for consumers.
AB - In this work, we identified the bacterial microbiota associated with farmed oystersin estuarine regions of four states in the north eastern region of Brazil. During the drought and rainy seasons, for eight months, twenty oysters were sampled seasonally from seven different marine farms. In the laboratory, DNA extraction, amplification, and sequencing of the 16S rRNA gene were performed to establish the taxonomic units. We identified 106 genera of bacteria belonging to 103 families, 70 orders, 39 classes, and 21 phyla. Out of the total, 40 of the genera represented bacteria potentially pathogenic to humans; of these, nine are known to cause foodborne diseases and six are potentially pathogenic to oysters. The most prevalent genera were Mycoplasma, Propionigenium, Psychrilyobacter, and Arcobacter. The results indicate the need for more systematic monitoring of bacteria of the genus Mycoplasma in oyster farming operations in the Brazilian north eastern region. Currently, Mycoplasma is not one of the microorganisms analysed and monitored by order of Brazilian legislation during the oyster production and/or commercialization process, even though this genus was the most prevalent at all sampling points and presents pathogenic potential both for oysters and for consumers.
KW - Contamination
KW - Cultivation
KW - Oyster farming
KW - Pathogenicity
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U2 - 10.1590/0001-3765202020180432
DO - 10.1590/0001-3765202020180432
M3 - Article (journal)
C2 - 32609272
AN - SCOPUS:85087528150
SN - 0001-3765
VL - 92
SP - 1
EP - 15
JO - Anais da Academia Brasileira de Ciencias
JF - Anais da Academia Brasileira de Ciencias
M1 - e20180432
ER -