Leuconostoc ficulneum sp. nov., a novel lactic acid bacterium isolated from a ripe fig, and reclassification of Lactobacillus fructosus as Leuconostoc fructosum comb. nov.

André Antunes, Fred A Rainey, M Fernanda Nobre, Peter Schumann, Ana Margarida Ferreira, Ana Ramos, Helena Santos, Milton S da Costa

Research output: Contribution to journalArticle

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Abstract

A novel, moderately halophilic bacterium was isolated from the brine–seawater interface of the Shaban Deep, northern Red Sea. A polyphasic approach was used for the taxonomic characterization of this isolate, with the phenotypic and phylogenetic data clearly showing the distinctiveness of this bacterium. Cells of isolate E1L3A were Gram-negative, monotrichous cocci that showed a remarkable physiological flexibility, as could be seen by the quite broad growth ranges for oxygen, temperature, NaCl, and, to a smaller degree, pH. In addition, it was able to grow from atmospheric pressure up to 15 MPa, making it a piezotolerant bacterium. Phylogenetically, strain E1L3A represents a new, deeply branching lineage within the c-Proteobacteria, as determined by 16S rRNA gene sequence analysis. No close relatives are known so far, with sequence similarity to other cultivated members of the c-Proteobacteria being lower than 88%. The creation of the new genus Salinisphaera and the new species Salinisphaera shabanensis (DSM 14853; JCM 11575) for this new and highly versatile microorganism is therefore proposed.
Original languageEnglish
Pages (from-to)647-655
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume52
Issue numberPt 2
DOIs
Publication statusPublished - Mar 2002

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Leuconostoc ficulneum
Leuconostoc fructosum
Leuconostoc
Ficus
Comb and Wattles
figs
Lactobacillus
taxonomic revisions
lactic acid bacteria
Proteobacteria
Lactic Acid
Bacteria
bacterium
new species
acid
bacteria
Atmospheric Pressure
Coccus
atmospheric pressure
rRNA Genes

Cite this

Antunes, André ; Rainey, Fred A ; Nobre, M Fernanda ; Schumann, Peter ; Ferreira, Ana Margarida ; Ramos, Ana ; Santos, Helena ; da Costa, Milton S. / Leuconostoc ficulneum sp. nov., a novel lactic acid bacterium isolated from a ripe fig, and reclassification of Lactobacillus fructosus as Leuconostoc fructosum comb. nov. In: International Journal of Systematic and Evolutionary Microbiology. 2002 ; Vol. 52, No. Pt 2. pp. 647-655.
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abstract = "A novel, moderately halophilic bacterium was isolated from the brine–seawater interface of the Shaban Deep, northern Red Sea. A polyphasic approach was used for the taxonomic characterization of this isolate, with the phenotypic and phylogenetic data clearly showing the distinctiveness of this bacterium. Cells of isolate E1L3A were Gram-negative, monotrichous cocci that showed a remarkable physiological flexibility, as could be seen by the quite broad growth ranges for oxygen, temperature, NaCl, and, to a smaller degree, pH. In addition, it was able to grow from atmospheric pressure up to 15 MPa, making it a piezotolerant bacterium. Phylogenetically, strain E1L3A represents a new, deeply branching lineage within the c-Proteobacteria, as determined by 16S rRNA gene sequence analysis. No close relatives are known so far, with sequence similarity to other cultivated members of the c-Proteobacteria being lower than 88{\%}. The creation of the new genus Salinisphaera and the new species Salinisphaera shabanensis (DSM 14853; JCM 11575) for this new and highly versatile microorganism is therefore proposed.",
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Leuconostoc ficulneum sp. nov., a novel lactic acid bacterium isolated from a ripe fig, and reclassification of Lactobacillus fructosus as Leuconostoc fructosum comb. nov. / Antunes, André; Rainey, Fred A; Nobre, M Fernanda; Schumann, Peter; Ferreira, Ana Margarida; Ramos, Ana; Santos, Helena; da Costa, Milton S.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 52, No. Pt 2, 03.2002, p. 647-655.

Research output: Contribution to journalArticle

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AB - A novel, moderately halophilic bacterium was isolated from the brine–seawater interface of the Shaban Deep, northern Red Sea. A polyphasic approach was used for the taxonomic characterization of this isolate, with the phenotypic and phylogenetic data clearly showing the distinctiveness of this bacterium. Cells of isolate E1L3A were Gram-negative, monotrichous cocci that showed a remarkable physiological flexibility, as could be seen by the quite broad growth ranges for oxygen, temperature, NaCl, and, to a smaller degree, pH. In addition, it was able to grow from atmospheric pressure up to 15 MPa, making it a piezotolerant bacterium. Phylogenetically, strain E1L3A represents a new, deeply branching lineage within the c-Proteobacteria, as determined by 16S rRNA gene sequence analysis. No close relatives are known so far, with sequence similarity to other cultivated members of the c-Proteobacteria being lower than 88%. The creation of the new genus Salinisphaera and the new species Salinisphaera shabanensis (DSM 14853; JCM 11575) for this new and highly versatile microorganism is therefore proposed.

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