Aims: To examine through the nasal potential difference (PD) profile, airway ion transport in moderately preterm infants (from 29 weeks gestation). Background: Despite recent advances, lung disease following premature birth remains a significant clinical problem. Animal and clinical studies suggest that airway ion transport has a critical role in the adaptation to air breathing in postnatal life. In particular, sodium absorption mediated through the epithelial sodium channel (ENaC) is a major factor in lung water clearance after birth.(1) The a-ENaC subunit m-RNA expression has been found to increase with gestational age.(2) Immature airway ion transport as a result of prematurity may therefore contribute to the respiratory morbidity in infants born moderately preterm. Methods: We studied 75 infants using a modified perfusion protocol suitable for neonates (healthy term infants n = 40; preterm 29-36 weeks gestation n = 35). We determined: 1.) Maximal baseline PD following perfusion of a physiological isotonic solution 2.) The change in PD following perfusion with amiloride 10-4 M (?amil, to assess ENaC activity) 3.) The change in PD, after perfusion with a solution low in Cl- (to assess the capacity for Cl- secretion). Results: Maximal baseline PDs are shown in graph 1. Four ventilated infants had maximal baseline PDs greater than -50mV. Amiloride perfusion resulted in substantial reduction in PD magnitude (median ?amil in pretem, 80%) and there was little capacity for chloride secretion in either cohort. □: ventilated male infants; ■: non-ventilated male infants ∆: ventilated female infants; ▲: non-ventilated female infants Conclusion: This is the first reported clinical study examining the postnatal airway ion transport pattern in moderately preterm infants and these data suggest these processes are well developed in these infants on day 1 of postnatal life. The findings support a normal switch from the fetal secretory pattern to a fluid absorptive state after birth in this cohort, similar to term infants. We found increased nasal PD in some ventilated preterm infants after birth. ?amil in these infants confirms that the increased PD principally reflects ENaC-mediated sodium absorption. The sodium hyperabsorption evident in some of the ventilated preterm infants may reflect a compensatory mechanism to clear fluid from the lungs.
|Publication status||Published - 2002|
|Event||European Society for Paediatric Research Annual Meeting - Utrecht, Netherlands|
Duration: 25 Dec 2002 → …
|Conference||European Society for Paediatric Research Annual Meeting|
|Period||25/12/02 → …|