Hydrogen sulfide enhances pancreatic β-cell differentiation from human tooth under normal and glucotoxic conditions

Ioan Valentin Matei, Hisataka Ii, Ken Yaegaki*

*Corresponding author for this work

Research output: Contribution to journalArticle (journal)peer-review

10 Citations (Scopus)

Abstract

Aim: Glucotoxicity obstructs pancreatic differentiation from adult stem cells. The aim was to develop a novel protocol for differentiation of dental pulp stem cells (DPSCs) into pancreatic β cells and determine the effect of H2S on glucotoxicity.

Materials & methods: DPSCs were differentiated with media containing 5.5 or 25.0 mM glucose, exposed to 1 ng/ml H2S. Glucotoxicity, expression of β-cell markers, INS, PDX1 and GLUT2, and PI3K/AKT pathway were assessed.

Results: H2S exposure increased insulin and C-peptide, and protected DPSC-derived pancreatic β-like cells from glucotoxicity and upregulated INS, PDX1 and GLUT2, and genes of PI3K/AKT pathway.

Conclusion: H2S improved effects of glucotoxicity on β-like cells via PI3K/AKT pathway. The protocol for pancreatic β-cell differentiation might have applications in regenerative medicine rather than swine pancreas transplantation.
Original languageEnglish
Pages (from-to)125-141
Number of pages16
JournalRegenerative Medicine
Volume12
Issue number2
DOIs
Publication statusPublished - 7 Dec 2016
Externally publishedYes

Keywords

  • glucotoxicity
  • hydrogen sulfide
  • pancreatic β-like cells

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