High Laccase Expression by Trametes versicolor in a Simulated Textile Effluent with Different Carbon Sources and PHs

Cristiane Ottoni, Marta Filipa Simões, Sara Fernandes, Cledir R. Santos Santos, Nelson Lima

Research output: Contribution to journalArticle

3 Citations (Scopus)
67 Downloads (Pure)

Abstract

Abstract: Textile effluents are highly polluting and have variable and complex compositions. They can be extremely complex, with high salt concentrations and alkaline pHs. A fixed-bed bioreactor was used in the present study to simulate a textile effluent treatment, where the white-rot fungus, Trametes versicolor, efficiently decolourised the azo dye Reactive Black 5 over 28 days. This occurred under high alkaline conditions, which is unusual, but advantageous, for successful decolourisation processes. Active dye decolourisation was maintained by operation in continuous culture. Colour was eliminated during the course of operation and maximum laccase (Lcc) activity (80.2 UL1) was detected after glycerol addition to the bioreactor. Lcc2 gene expression was evaluated with different carbon sources and pH values based on reverse transcriptase-PCR (polymerase chain reaction). Glycerol was shown to promote the highest lcc2 expression at pH 5.5, followed by sucrose and then glucose. The highest levels of expression occurred between three and four days, which corroborate the maximum Lcc activity observed for sucrose and glycerol on the bioreactor. These results give new insights into the use of T. versicolor in textile dye wastewater treatment with high pHs.
Original languageEnglish
Pages (from-to)1-10
JournalInternational Journal of Environmental Research and Public Health
Volume13
Issue number778
DOIs
Publication statusPublished - 2 Aug 2016

Fingerprint Dive into the research topics of 'High Laccase Expression by Trametes versicolor in a Simulated Textile Effluent with Different Carbon Sources and PHs'. Together they form a unique fingerprint.

  • Cite this