TY - JOUR
T1 - High Laccase Expression by Trametes versicolor in a
Simulated Textile Effluent with Different Carbon
Sources and PHs
AU - Ottoni, Cristiane
AU - Simões, Marta Filipa
AU - Fernandes, Sara
AU - Santos, Cledir R. Santos
AU - Lima, Nelson
PY - 2016/8/2
Y1 - 2016/8/2
N2 - Abstract: Textile effluents are highly polluting and have variable and complex compositions. They can
be extremely complex, with high salt concentrations and alkaline pHs. A fixed-bed bioreactor
was used in the present study to simulate a textile effluent treatment, where the white-rot fungus,
Trametes versicolor, efficiently decolourised the azo dye Reactive Black 5 over 28 days. This occurred
under high alkaline conditions, which is unusual, but advantageous, for successful decolourisation
processes. Active dye decolourisation was maintained by operation in continuous culture. Colour was
eliminated during the course of operation and maximum laccase (Lcc) activity (80.2 UL1) was
detected after glycerol addition to the bioreactor. Lcc2 gene expression was evaluated with different
carbon sources and pH values based on reverse transcriptase-PCR (polymerase chain reaction).
Glycerol was shown to promote the highest lcc2 expression at pH 5.5, followed by sucrose and then
glucose. The highest levels of expression occurred between three and four days, which corroborate
the maximum Lcc activity observed for sucrose and glycerol on the bioreactor. These results give
new insights into the use of T. versicolor in textile dye wastewater treatment with high pHs.
AB - Abstract: Textile effluents are highly polluting and have variable and complex compositions. They can
be extremely complex, with high salt concentrations and alkaline pHs. A fixed-bed bioreactor
was used in the present study to simulate a textile effluent treatment, where the white-rot fungus,
Trametes versicolor, efficiently decolourised the azo dye Reactive Black 5 over 28 days. This occurred
under high alkaline conditions, which is unusual, but advantageous, for successful decolourisation
processes. Active dye decolourisation was maintained by operation in continuous culture. Colour was
eliminated during the course of operation and maximum laccase (Lcc) activity (80.2 UL1) was
detected after glycerol addition to the bioreactor. Lcc2 gene expression was evaluated with different
carbon sources and pH values based on reverse transcriptase-PCR (polymerase chain reaction).
Glycerol was shown to promote the highest lcc2 expression at pH 5.5, followed by sucrose and then
glucose. The highest levels of expression occurred between three and four days, which corroborate
the maximum Lcc activity observed for sucrose and glycerol on the bioreactor. These results give
new insights into the use of T. versicolor in textile dye wastewater treatment with high pHs.
U2 - 10.3390/ijerph13080778
DO - 10.3390/ijerph13080778
M3 - Article (journal)
SN - 1660-4601
VL - 13
SP - 1
EP - 10
JO - International Journal of Environmental Research and Public Health
JF - International Journal of Environmental Research and Public Health
IS - 778
ER -