Fate of cystatin C lacking the leader sequence in RPE cells

Luminita Paraoan, Ian Grierson, B Edward H Maden

Research output: Contribution to journalArticle (journal)peer-review

17 Citations (Scopus)

Abstract

The study made use of transiently transfected RPE cells expressing mature cystatin C (lacking the 26 amino acids leader sequence of the precursor cystatin C) fused to enhanced green fluorescent protein (EGFP) and, in control transfections, EGFP on its own. The findings demonstrate that 'leaderless' cystatin C is not processed through the secretory pathway of RPE cells. Since a polymorphism in the leader sequence has recently been associated with increased risk for development of exudative age-related macular degeneration (AMD), the present findings lend support to the hypothesis that impairment of function of the leader sequence may contribute to the aetiology of exudative AMD.

Original languageEnglish
Pages (from-to)753-6
Number of pages4
JournalExperimental Eye Research
Volume76
Issue number6
DOIs
Publication statusPublished - 1 Jun 2003

Keywords

  • 5' Untranslated Regions
  • Cells, Cultured
  • Cystatin C
  • Cystatins/genetics
  • Green Fluorescent Proteins
  • Humans
  • Luminescent Proteins/genetics
  • Macular Degeneration/metabolism
  • Microscopy, Confocal
  • Microscopy, Fluorescence
  • Pigment Epithelium of Eye/metabolism
  • Retina/metabolism
  • Transfection

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