Fate of cystatin C lacking the leader sequence in RPE cells

Luminita Paraoan; Ian Grierson; B Edward H Maden

doi:10.1016/s0014-4835(03)00061-7

Fate of cystatin C lacking the leader sequence in RPE cells

Luminita Paraoan, Ian Grierson, B Edward H Maden

Biology

Research output: Contribution to journal › Article (journal) › peer-review

18 Citations (Scopus)

Abstract

The study made use of transiently transfected RPE cells expressing mature cystatin C (lacking the 26 amino acids leader sequence of the precursor cystatin C) fused to enhanced green fluorescent protein (EGFP) and, in control transfections, EGFP on its own. The findings demonstrate that 'leaderless' cystatin C is not processed through the secretory pathway of RPE cells. Since a polymorphism in the leader sequence has recently been associated with increased risk for development of exudative age-related macular degeneration (AMD), the present findings lend support to the hypothesis that impairment of function of the leader sequence may contribute to the aetiology of exudative AMD.

Original language	English
Pages (from-to)	753-6
Number of pages	4
Journal	Experimental Eye Research
Volume	76
Issue number	6
DOIs	https://doi.org/10.1016/s0014-4835(03)00061-7
Publication status	Published - 1 Jun 2003

Keywords

5' Untranslated Regions
Cells, Cultured
Cystatin C
Cystatins/genetics
Green Fluorescent Proteins
Humans
Luminescent Proteins/genetics
Macular Degeneration/metabolism
Microscopy, Confocal
Microscopy, Fluorescence
Pigment Epithelium of Eye/metabolism
Retina/metabolism
Transfection

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title = "Fate of cystatin C lacking the leader sequence in RPE cells",

abstract = "The study made use of transiently transfected RPE cells expressing mature cystatin C (lacking the 26 amino acids leader sequence of the precursor cystatin C) fused to enhanced green fluorescent protein (EGFP) and, in control transfections, EGFP on its own. The findings demonstrate that 'leaderless' cystatin C is not processed through the secretory pathway of RPE cells. Since a polymorphism in the leader sequence has recently been associated with increased risk for development of exudative age-related macular degeneration (AMD), the present findings lend support to the hypothesis that impairment of function of the leader sequence may contribute to the aetiology of exudative AMD.",

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doi = "10.1016/s0014-4835(03)00061-7",

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T1 - Fate of cystatin C lacking the leader sequence in RPE cells

AU - Paraoan, Luminita

AU - Grierson, Ian

AU - Maden, B Edward H

PY - 2003/6/1

Y1 - 2003/6/1

N2 - The study made use of transiently transfected RPE cells expressing mature cystatin C (lacking the 26 amino acids leader sequence of the precursor cystatin C) fused to enhanced green fluorescent protein (EGFP) and, in control transfections, EGFP on its own. The findings demonstrate that 'leaderless' cystatin C is not processed through the secretory pathway of RPE cells. Since a polymorphism in the leader sequence has recently been associated with increased risk for development of exudative age-related macular degeneration (AMD), the present findings lend support to the hypothesis that impairment of function of the leader sequence may contribute to the aetiology of exudative AMD.

AB - The study made use of transiently transfected RPE cells expressing mature cystatin C (lacking the 26 amino acids leader sequence of the precursor cystatin C) fused to enhanced green fluorescent protein (EGFP) and, in control transfections, EGFP on its own. The findings demonstrate that 'leaderless' cystatin C is not processed through the secretory pathway of RPE cells. Since a polymorphism in the leader sequence has recently been associated with increased risk for development of exudative age-related macular degeneration (AMD), the present findings lend support to the hypothesis that impairment of function of the leader sequence may contribute to the aetiology of exudative AMD.

KW - 5' Untranslated Regions

KW - Cells, Cultured

KW - Cystatin C

KW - Cystatins/genetics

KW - Green Fluorescent Proteins

KW - Humans

KW - Luminescent Proteins/genetics

KW - Macular Degeneration/metabolism

KW - Microscopy, Confocal

KW - Microscopy, Fluorescence

KW - Pigment Epithelium of Eye/metabolism

KW - Retina/metabolism

KW - Transfection

U2 - 10.1016/s0014-4835(03)00061-7

DO - 10.1016/s0014-4835(03)00061-7

M3 - Article (journal)

C2 - 12742358

SN - 0014-4835

VL - 76

SP - 753

EP - 756

JO - Experimental Eye Research

JF - Experimental Eye Research

IS - 6

ER -

Fate of cystatin C lacking the leader sequence in RPE cells

Abstract

Keywords

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