Evaluation of telomerase activity in bronchial lavage as a potential diagnostic marker for malignant lung disease

G Xinarianos; F M Scott; T Liloglou; W Prime; L Turnbull; M Walshaw; J K Field

doi:10.1016/s0169-5002(99)00111-7

Evaluation of telomerase activity in bronchial lavage as a potential diagnostic marker for malignant lung disease

G Xinarianos, F M Scott, T Liloglou, W Prime, L Turnbull, M Walshaw, J K Field

Medical School

Research output: Contribution to journal › Article (journal) › peer-review

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Abstract

Telomerase is a ribonucleoprotein DNA polymerase that maintains the telomeric region of chromosomes lost during successive rounds of cell division. We used the telomeric repeat amplification protocol (TRAP) assay to examine telomerase activity in bronchial lavage (BL) samples from individuals undergoing diagnosis of lung cancer. Telomerase activity was detected in 17 (47%) of 36 samples examined. In particular, 16 (70%) of 23 BL specimens obtained from lung cancer patients showed detectable telomerase activity, while only 1 of 13 (8%) specimens obtained from patients without lung cancer demonstrated activity (P=0.00038). Moreover, 9 (90%) of 10 BL specimens, which were cytologically positive for lung cancer, were also positive for telomerase activity, while 7 (54%) of 13 cytologically negative BL specimens for lung cancer showed detectable telomerase activity. Detection of telomerase activity combined with cytology were able to identify 17 (74%) of 23 lung cancer cases whereas cytology alone identified 10 (43%) of 23 such cases (P=0.035). Our findings indicate that telomerase is a specific marker for malignant lung disease and a potential complementary tool to cytology in the diagnosis of certain lung cancer cases.

Original language	English
Pages (from-to)	37-42
Number of pages	6
Journal	Lung Cancer
Volume	28
Issue number	1
DOIs	https://doi.org/10.1016/s0169-5002(99)00111-7
Publication status	Published - 30 Apr 2000

Keywords

Biomarkers, Tumor/analysis
Bronchoalveolar Lavage Fluid/chemistry
Carcinoma, Non-Small-Cell Lung/enzymology
DNA, Neoplasm/analysis
Diagnosis, Differential
Genes, ras/genetics
Humans
Lung Neoplasms/enzymology
Polymerase Chain Reaction
Telomerase/analysis
Tumor Cells, Cultured

UN SDGs

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title = "Evaluation of telomerase activity in bronchial lavage as a potential diagnostic marker for malignant lung disease",

abstract = "Telomerase is a ribonucleoprotein DNA polymerase that maintains the telomeric region of chromosomes lost during successive rounds of cell division. We used the telomeric repeat amplification protocol (TRAP) assay to examine telomerase activity in bronchial lavage (BL) samples from individuals undergoing diagnosis of lung cancer. Telomerase activity was detected in 17 (47%) of 36 samples examined. In particular, 16 (70%) of 23 BL specimens obtained from lung cancer patients showed detectable telomerase activity, while only 1 of 13 (8%) specimens obtained from patients without lung cancer demonstrated activity (P=0.00038). Moreover, 9 (90%) of 10 BL specimens, which were cytologically positive for lung cancer, were also positive for telomerase activity, while 7 (54%) of 13 cytologically negative BL specimens for lung cancer showed detectable telomerase activity. Detection of telomerase activity combined with cytology were able to identify 17 (74%) of 23 lung cancer cases whereas cytology alone identified 10 (43%) of 23 such cases (P=0.035). Our findings indicate that telomerase is a specific marker for malignant lung disease and a potential complementary tool to cytology in the diagnosis of certain lung cancer cases.",

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author = "G Xinarianos and Scott, {F M} and T Liloglou and W Prime and L Turnbull and M Walshaw and Field, {J K}",

year = "2000",

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doi = "10.1016/s0169-5002(99)00111-7",

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T1 - Evaluation of telomerase activity in bronchial lavage as a potential diagnostic marker for malignant lung disease

AU - Xinarianos, G

AU - Scott, F M

AU - Liloglou, T

AU - Prime, W

AU - Turnbull, L

AU - Walshaw, M

AU - Field, J K

PY - 2000/4/30

Y1 - 2000/4/30

N2 - Telomerase is a ribonucleoprotein DNA polymerase that maintains the telomeric region of chromosomes lost during successive rounds of cell division. We used the telomeric repeat amplification protocol (TRAP) assay to examine telomerase activity in bronchial lavage (BL) samples from individuals undergoing diagnosis of lung cancer. Telomerase activity was detected in 17 (47%) of 36 samples examined. In particular, 16 (70%) of 23 BL specimens obtained from lung cancer patients showed detectable telomerase activity, while only 1 of 13 (8%) specimens obtained from patients without lung cancer demonstrated activity (P=0.00038). Moreover, 9 (90%) of 10 BL specimens, which were cytologically positive for lung cancer, were also positive for telomerase activity, while 7 (54%) of 13 cytologically negative BL specimens for lung cancer showed detectable telomerase activity. Detection of telomerase activity combined with cytology were able to identify 17 (74%) of 23 lung cancer cases whereas cytology alone identified 10 (43%) of 23 such cases (P=0.035). Our findings indicate that telomerase is a specific marker for malignant lung disease and a potential complementary tool to cytology in the diagnosis of certain lung cancer cases.

AB - Telomerase is a ribonucleoprotein DNA polymerase that maintains the telomeric region of chromosomes lost during successive rounds of cell division. We used the telomeric repeat amplification protocol (TRAP) assay to examine telomerase activity in bronchial lavage (BL) samples from individuals undergoing diagnosis of lung cancer. Telomerase activity was detected in 17 (47%) of 36 samples examined. In particular, 16 (70%) of 23 BL specimens obtained from lung cancer patients showed detectable telomerase activity, while only 1 of 13 (8%) specimens obtained from patients without lung cancer demonstrated activity (P=0.00038). Moreover, 9 (90%) of 10 BL specimens, which were cytologically positive for lung cancer, were also positive for telomerase activity, while 7 (54%) of 13 cytologically negative BL specimens for lung cancer showed detectable telomerase activity. Detection of telomerase activity combined with cytology were able to identify 17 (74%) of 23 lung cancer cases whereas cytology alone identified 10 (43%) of 23 such cases (P=0.035). Our findings indicate that telomerase is a specific marker for malignant lung disease and a potential complementary tool to cytology in the diagnosis of certain lung cancer cases.

KW - Biomarkers, Tumor/analysis

KW - Bronchoalveolar Lavage Fluid/chemistry

KW - Carcinoma, Non-Small-Cell Lung/enzymology

KW - DNA, Neoplasm/analysis

KW - Diagnosis, Differential

KW - Genes, ras/genetics

KW - Humans

KW - Lung Neoplasms/enzymology

KW - Polymerase Chain Reaction

KW - Telomerase/analysis

KW - Tumor Cells, Cultured

U2 - 10.1016/s0169-5002(99)00111-7

DO - 10.1016/s0169-5002(99)00111-7

M3 - Article (journal)

C2 - 10704707

SN - 0169-5002

VL - 28

SP - 37

EP - 42

JO - Lung Cancer

JF - Lung Cancer

IS - 1

ER -

Evaluation of telomerase activity in bronchial lavage as a potential diagnostic marker for malignant lung disease

Abstract

Keywords

UN SDGs

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