Effective microorganism – X attenuates circulating superoxide dismutase following an acute bout of intermittent running in hot, humid conditions

L. Taylor, B.J. Lee, O.R. Gibson, A.W. Midgley, P. Watt, A. Mauger, P. Castle

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This study determined the effectiveness of antioxidant supplementation on high-intensity exercise-heat stress. Six males completed a high-intensity running protocol twice in temperate conditions (TEMP; 20.4°C), and twice in hot conditions (HOT; 34.7°C). Trials were completed following7 days supplementation with 70 ml·day−1 effective microorganism-X (EM-X; TEMPEMX or HOTEMX) or placebo (TEMPPLA or HOTPLA). Plasma extracellular Hsp72 (eHsp72) and superoxide dismutase (SOD) were measured by ELISA. eHsp72 and SOD increased pre-post exercise (p < 0.001), with greater eHsp72 (p < 0.001) increases observed in HOT (+1.5 ng·ml−1) compared to TEMP (+0.8 ng·ml−1). EM-X did not influence eHsp72 (p > 0.05). Greater (p < 0.001) SOD increases were observed in HOT (+0.22 U·ml−1) versus TEMP (+0.10 U·ml−1) with SOD reduced in HOTEMX versus HOTPLA (p = 0.001). Physiological and perceptual responses were all greater (p < 0.001) in HOT versus TEMP conditions, with no difference followed EM-X (p > 0.05). EM-X supplementation attenuated the SOD increases following HOT, potentiating its application as an ergogenic aid to ameliorate oxidative stress.
Original languageUndefined/Unknown
Pages (from-to)130-144
JournalResearch in Sports Medicine
Issue number2
Early online date31 Mar 2016
Publication statusPublished - 2016


  • heat shock proteins
  • oxidative stress
  • redox balance
  • repeated sprint
  • exercise
  • HIT
  • human

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