Development of a real-time PCR assay for the detection of the golden mussel (limnoperna fortunei, mytilidae) in environmental samples

Marcio Roberto Pie*, Patrícia R. Ströher, André O. Agostinis, Ricardo Belmonte Lopes, Michelle Z.Tadra Sfeir, Antonio Ostrensky

*Corresponding author for this work

Research output: Contribution to journalArticle (journal)peer-review

11 Citations (Scopus)
4 Downloads (Pure)

Abstract

The golden mussel, Limnoperna fortunei, is among the most devastating invasive species in freshwater habitats worldwide, leading to severe environmental disturbances and economic losses. Therefore, management efforts would be greatly improved by methods that efficiently detect and quantify the abundance of the golden mussel in freshwater habitats, particularly in early stages of colonization. In this study, we describe a highly-sensitive real-time PCR assay targeting a 100-bp region of the COI mitochondrial gene of the golden mussel. The method was able to detect as little as 0.225 pg of target DNA. This assay represents an important contribution to surveillance methods, as well as to optimize field measures to contain and manage populations of the golden mussel in its introduced range.

Original languageEnglish
Pages (from-to)1041-1045
Number of pages5
JournalAnais da Academia Brasileira de Ciencias
Volume89
Issue number2
DOIs
Publication statusPublished - 2017

Keywords

  • EDNA
  • Environmental DNA
  • Invasive species
  • Molecular identification

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