Detection of Konjac glucomannan by immunoassay

I.P. Hurley; N.A. Pickles; H. Qin; H. Elyse Ireland; R.C. Coleman; B.N. Tosun; Z. Buyuktuncer; J.H.H. Williams

doi:10.1111/j.1365-2621.2010.02281.x

Detection of Konjac glucomannan by immunoassay

I.P. Hurley, N.A. Pickles, H. Qin, H. Elyse Ireland, R.C. Coleman, B.N. Tosun, Z. Buyuktuncer, J.H.H. Williams

Arts & Sciences Faculty Office

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3 Citations (Scopus)

Abstract

Konjac glucomannan is a hydrocolloid that has been used in food applications. The European ban on the use of Konjac glucomannan means that the detection and analysis has potential applications in the food industry, particularly detection of food adulteration. The aim of this work was to develop an assay capable of detecting Konjac glucomannan as an isolated sample and within food matrices. An indirect competitive ELISA was developed utilising a polyclonal antibody raised against Konjac glucomannan. The ELISA was found to be specific for Konjac glucomannan and sensitive, with a detection limit of 0.1 ng mL−1. Increasing salt concentration and freeze/thaw cycles did not affect the performance of the assay. The ELISA was able to detect Konjac glucomannan in admixtures with other gums and also in confectionery that had been spiked with Konjac glucomannan. The ELISA has potential as a kit for the differentiation of Konjac glucomannan from other hydrocolloids and detection in food.

Original language	English
Pages (from-to)	1410-1416
Number of pages	7
Journal	International Journal of Food Science and Technology
Volume	45
Issue number	7
DOIs	https://doi.org/10.1111/j.1365-2621.2010.02281.x
Publication status	Published - 21 Jun 2010

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title = "Detection of Konjac glucomannan by immunoassay",

abstract = "Konjac glucomannan is a hydrocolloid that has been used in food applications. The European ban on the use of Konjac glucomannan means that the detection and analysis has potential applications in the food industry, particularly detection of food adulteration. The aim of this work was to develop an assay capable of detecting Konjac glucomannan as an isolated sample and within food matrices. An indirect competitive ELISA was developed utilising a polyclonal antibody raised against Konjac glucomannan. The ELISA was found to be specific for Konjac glucomannan and sensitive, with a detection limit of 0.1 ng mL−1. Increasing salt concentration and freeze/thaw cycles did not affect the performance of the assay. The ELISA was able to detect Konjac glucomannan in admixtures with other gums and also in confectionery that had been spiked with Konjac glucomannan. The ELISA has potential as a kit for the differentiation of Konjac glucomannan from other hydrocolloids and detection in food.",

author = "I.P. Hurley and N.A. Pickles and H. Qin and {Elyse Ireland}, H. and R.C. Coleman and B.N. Tosun and Z. Buyuktuncer and J.H.H. Williams",

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T1 - Detection of Konjac glucomannan by immunoassay

AU - Hurley, I.P.

AU - Pickles, N.A.

AU - Qin, H.

AU - Elyse Ireland, H.

AU - Coleman, R.C.

AU - Tosun, B.N.

AU - Buyuktuncer, Z.

AU - Williams, J.H.H.

N1 - Export Date: 29 May 2024; Cited By: 2

PY - 2010/6/21

Y1 - 2010/6/21

N2 - Konjac glucomannan is a hydrocolloid that has been used in food applications. The European ban on the use of Konjac glucomannan means that the detection and analysis has potential applications in the food industry, particularly detection of food adulteration. The aim of this work was to develop an assay capable of detecting Konjac glucomannan as an isolated sample and within food matrices. An indirect competitive ELISA was developed utilising a polyclonal antibody raised against Konjac glucomannan. The ELISA was found to be specific for Konjac glucomannan and sensitive, with a detection limit of 0.1 ng mL−1. Increasing salt concentration and freeze/thaw cycles did not affect the performance of the assay. The ELISA was able to detect Konjac glucomannan in admixtures with other gums and also in confectionery that had been spiked with Konjac glucomannan. The ELISA has potential as a kit for the differentiation of Konjac glucomannan from other hydrocolloids and detection in food.

AB - Konjac glucomannan is a hydrocolloid that has been used in food applications. The European ban on the use of Konjac glucomannan means that the detection and analysis has potential applications in the food industry, particularly detection of food adulteration. The aim of this work was to develop an assay capable of detecting Konjac glucomannan as an isolated sample and within food matrices. An indirect competitive ELISA was developed utilising a polyclonal antibody raised against Konjac glucomannan. The ELISA was found to be specific for Konjac glucomannan and sensitive, with a detection limit of 0.1 ng mL−1. Increasing salt concentration and freeze/thaw cycles did not affect the performance of the assay. The ELISA was able to detect Konjac glucomannan in admixtures with other gums and also in confectionery that had been spiked with Konjac glucomannan. The ELISA has potential as a kit for the differentiation of Konjac glucomannan from other hydrocolloids and detection in food.

U2 - 10.1111/j.1365-2621.2010.02281.x

DO - 10.1111/j.1365-2621.2010.02281.x

M3 - Article (journal)

SN - 0950-5423

VL - 45

SP - 1410

EP - 1416

JO - International Journal of Food Science and Technology

JF - International Journal of Food Science and Technology

IS - 7

ER -

Detection of Konjac glucomannan by immunoassay

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