Comparison of nanocomplexes with branched and linear peptides for siRNA delivery

Aristides D Tagalakis, Luisa Saraiva, David McCarthy, Kenth T Gustafsson, Stephen L Hart

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Efficient delivery of small interfering RNA (siRNA) remains the greatest technological barrier to the clinical implementation of RNA interference strategies. We are investigating the relationship between the biophysical properties of siRNA nanocomplexes and their transfection efficiency as an approach to the generation of improved formulations. Peptide-based formulations are of great interest, and so in this study we have compared nanocomplex formulations for siRNA delivery containing linear and branched oligolysine or oligoarginine peptides. Peptides were combined with cationic liposomes in siRNA formulations and compared for transfection efficiency, siRNA packaging efficiency, biophysical properties, and particle stability. Nanocomplexes containing linear peptides were more condensed and stable than branched peptide formulations; however, their silencing activity was lower, suggesting that their greater stability might limit siRNA release within the cell. Thus, differences in transfection appeared to be associated with differences in packaging and stability, indicating the importance of optimizing this feature in siRNA nanocomplexes.

Original languageEnglish
Pages (from-to)761-70
Number of pages10
JournalBiomacromolecules
Volume14
Issue number3
DOIs
Publication statusPublished - 11 Mar 2013

Fingerprint

RNA
Peptides
Small Interfering RNA
Packaging
Liposomes

Keywords

  • Animals
  • Cations
  • Cell Line, Tumor
  • Cell Proliferation
  • Drug Delivery Systems
  • Liposomes
  • Luciferases/analysis
  • Mice
  • Microscopy, Confocal
  • Microscopy, Electron, Transmission
  • Nanostructures/chemistry
  • Particle Size
  • Peptides/chemistry
  • RNA Interference
  • RNA, Small Interfering/genetics
  • Transfection

Cite this

Tagalakis, Aristides D ; Saraiva, Luisa ; McCarthy, David ; Gustafsson, Kenth T ; Hart, Stephen L. / Comparison of nanocomplexes with branched and linear peptides for siRNA delivery. In: Biomacromolecules. 2013 ; Vol. 14, No. 3. pp. 761-70.
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Comparison of nanocomplexes with branched and linear peptides for siRNA delivery. / Tagalakis, Aristides D; Saraiva, Luisa; McCarthy, David; Gustafsson, Kenth T; Hart, Stephen L.

In: Biomacromolecules, Vol. 14, No. 3, 11.03.2013, p. 761-70.

Research output: Contribution to journalArticle

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T1 - Comparison of nanocomplexes with branched and linear peptides for siRNA delivery

AU - Tagalakis, Aristides D

AU - Saraiva, Luisa

AU - McCarthy, David

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AU - Hart, Stephen L

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KW - Microscopy, Electron, Transmission

KW - Nanostructures/chemistry

KW - Particle Size

KW - Peptides/chemistry

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