Characterization of cyclic adenine dinucleotide phosphate ribose levels in human spermatozoa

Richard A Billington, Claire Harper, Elisa A Bellomo, Steve Publicover, Christopher L R Barratt, Armando A Genazzani

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

OBJECTIVE: To determine the presence of the Ca2+-releasing pyridine nucleotide derivative, cyclic adenine dinucleotide phosphate ribose (cADPR), in human spermatozoa and to investigate its role in progesterone-induced Ca2+ oscillations in spermatozoa.

DESIGN: Biochemical investigation on human spermatozoa from healthy volunteers.

SETTING: Healthy volunteers in an academic research environment.

PATIENT(S): Ten volunteers.

INTERVENTION(S): None.

MAIN OUTCOME MEASURE(S): The cADPR levels.

RESULT(S): Human spermatozoa contain micromolar concentrations of cADPR that do not change significantly during sperm capacitation. An active synthetic machinery for cADPR is present in human spermatozoa, whereas degradation activity is minimal. Although progesterone-induced Ca2+ oscillations are dependent on the ryanodine receptor, they are unaffected by cADPR antagonists.

CONCLUSION(S): It appears that cADPR does not to play a role in Ca2+ oscillations in spermatozoa, but the presence of high concentrations of cADPR suggests that, instead, it may be introduced into the egg at fertilization and play a role in the Ca2+ transient immediately following sperm-egg fusion.

Original languageEnglish
Pages (from-to)891-8
Number of pages8
JournalFertility and Sterility
Volume86
Issue number4
Early online date9 Aug 2006
DOIs
Publication statusPublished - 30 Oct 2006

Keywords

  • Calcium/metabolism
  • Calcium Signaling/physiology
  • Cells, Cultured
  • Cyclic ADP-Ribose/metabolism
  • Humans
  • Male
  • Sperm Capacitation/physiology
  • Spermatozoa/metabolism

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