CapA, an autotransporter protein of Campylobacter jejuni, mediates association with human epithelial cells and colonization of the chicken gut

Sami S A Ashgar, Neil J Oldfield, Karl G Wooldridge, Michael A Jones, Greg J Irving, David P J Turner, Dlawer A A Ala'Aldeen

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

Two putative autotransporter proteins, CapA and CapB, were identified in silico from the genome sequence of Campylobacter jejuni NCTC11168. The genes encoding each protein contain homopolymeric tracts, suggestive of phase variation mediated by a slipped-strand mispairing mechanism; in each case the gene sequence contained frameshifts at these positions. The C-terminal two-thirds of the two genes, as well as a portion of the predicted signal peptides, were identical; the remaining N-terminal portions were gene specific. Both genes were cloned and expressed; recombinant polypeptides were purified and used to raise rabbit polyclonal monospecific antisera. Using immunoblotting, expression of the ca.116-kDa CapA protein was demonstrated for in vitro-grown cells of strain NCTC11168, for 4 out of 11 recent human fecal isolates, and for 2 out of 8 sequence-typed strains examined. Expression of CapB was not detected for any of the strains tested. Surface localization of CapA was demonstrated by subcellular fractionation and immunogold electron microscopy. Export of CapA was inhibited by globomycin, reinforcing the bioinformatic prediction that the protein is a lipoprotein. A capA insertion mutant had a significantly reduced capacity for association with and invasion of Caco-2 cells and failed to colonize and persist in chickens, indicating that CapA plays a role in host association and colonization by Campylobacter. In view of this demonstrated role, we propose that CapA stands for Campylobacter adhesion protein A.

Original languageEnglish
Pages (from-to)1856-65
Number of pages10
JournalJournal of Bacteriology
Volume189
Issue number5
DOIs
Publication statusPublished - 31 Mar 2007

Keywords

  • Animals
  • Bacterial Adhesion
  • Bacterial Proteins/genetics
  • Campylobacter jejuni/pathogenicity
  • Chickens/microbiology
  • Cloning, Molecular
  • Epithelial Cells/microbiology
  • Heat-Shock Proteins/genetics
  • Humans
  • Intestines/microbiology

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