Blocking variant surface glycoprotein synthesis alters endoplasmic reticulum exit sites/Golgi homeostasis in Trypanosoma brucei

CHER-PHENG OOI, Terry K Smith, Eva Gluenz, Nadina Wand, Sue Vaughan, Gloria Rudenko

Research output: Contribution to journalArticle

1 Citation (Scopus)
7 Downloads (Pure)

Abstract

The predominant secretory cargo of bloodstream form Trypanosoma brucei is variant surface glycoprotein (VSG), comprising ~10% total protein and forming a dense protective layer. Blocking VSG translation using Morpholino oligonucleotides triggered a precise pre-cytokinesis arrest. We investigated the effect of blocking VSG synthesis on the secretory pathway. The number of Golgi decreased, particularly in post-mitotic cells, from 3.5 ± 0.6 to 2.0 ± 0.04 per cell. Similarly, the number of endoplasmic reticulum exit sites (ERES) in post-mitotic cells dropped from 3.9 ± 0.6 to 2.7 ± 0.1 eight hours after blocking VSG synthesis. The secretory pathway was still functional in these stalled cells, as monitored using Cathepsin L. Rates of phospholipid and glycosylphosphatidylinositol-anchor biosynthesis remained relatively unaffected, except for the level of sphingomyelin which increased. However, both endoplasmic reticulum and Golgi morphology became distorted, with the Golgi cisternae becoming significantly dilated, particularly at the trans-face. Membrane accumulation in these structures is possibly caused by reduced budding of nascent vesicles due to the drastic reduction in the total amount of secretory cargo, that is, VSG. These data argue that the total flux of secretory cargo impacts upon the biogenesis and maintenance of secretory structures and organelles in T. brucei, including the ERES and Golgi.
Original languageEnglish
Pages (from-to)391 - 405
Number of pages15
JournalTraffic
Volume19
Issue number6
Early online date13 Mar 2018
DOIs
Publication statusPublished - 1 Jun 2018

Keywords

  • ER exit site
  • Golgi biogenesis
  • membrane metabolism
  • secretory pathway
  • Trypanosoma brucei,
  • variant surface glycoprotein
  • Trypanosoma brucei

Fingerprint Dive into the research topics of 'Blocking variant surface glycoprotein synthesis alters endoplasmic reticulum exit sites/Golgi homeostasis in Trypanosoma brucei'. Together they form a unique fingerprint.

Cite this