TY - JOUR
T1 - Bioprospecting highly diverse endophytic Pestalotiopsis spp. with antibacterial properties from Maytenus ilicifolia, a medicinal plant from Brazil
AU - Gomes-Figueiredo, Josiane
AU - Pimentel, Ida C.
AU - Vicente, Vânia A.
AU - Pie, Marcio R.
AU - Kava-Cordeiro, Vanessa
AU - Galli-Terasawa, Lygia
AU - Pereira, José O.
AU - De Souza, Antonie Q.L.
AU - Glienke, Chirlei
PY - 2007/10/1
Y1 - 2007/10/1
N2 - Thirteen endophytic fungal strains of the genus Pestalotiopsis were isolated from the medicinal plant Maytenus ilicifolia Mart. ex. Reiss (commonly known as "espinheira santa") and their antimicrobial properties were investigated. Two isolates were successful in inhibiting the growth of the tested microorganisms (Escherichia coli, Klebsiella pneumoniae, Micrococcus luteus, Staphylococcus aureus, and methicillin-resistant Staphylococcus aureus (MRSA)) using the technique of bioautographic thin-layer chromatography (TLC) agar overlay assay. An analysis based on a polyphasic approach integrating taxonomic information, morphological traits, RAPD markers, and the sequencing of the ITS1-5.8S-ITS2 of the rDNA led to the assignment of the isolates as belonging to the species Pestalotiopsis microspora, Pestalotiopsis vismiae, and Pestalotiopsis leucothoes. Therefore, the present study presents a new approach to the study of endophytic fungi of the genus Pestalotiopsis.
AB - Thirteen endophytic fungal strains of the genus Pestalotiopsis were isolated from the medicinal plant Maytenus ilicifolia Mart. ex. Reiss (commonly known as "espinheira santa") and their antimicrobial properties were investigated. Two isolates were successful in inhibiting the growth of the tested microorganisms (Escherichia coli, Klebsiella pneumoniae, Micrococcus luteus, Staphylococcus aureus, and methicillin-resistant Staphylococcus aureus (MRSA)) using the technique of bioautographic thin-layer chromatography (TLC) agar overlay assay. An analysis based on a polyphasic approach integrating taxonomic information, morphological traits, RAPD markers, and the sequencing of the ITS1-5.8S-ITS2 of the rDNA led to the assignment of the isolates as belonging to the species Pestalotiopsis microspora, Pestalotiopsis vismiae, and Pestalotiopsis leucothoes. Therefore, the present study presents a new approach to the study of endophytic fungi of the genus Pestalotiopsis.
KW - Endophytic fungi
KW - Espinheira santa
KW - Pestalotiopsis
KW - Polyphasic approach
KW - RAPD
UR - http://www.scopus.com/inward/record.url?scp=36549067893&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=36549067893&partnerID=8YFLogxK
U2 - 10.1139/W07-078
DO - 10.1139/W07-078
M3 - Article (journal)
C2 - 18026204
AN - SCOPUS:36549067893
SN - 0008-4166
VL - 53
SP - 1123
EP - 1132
JO - Canadian Journal of Microbiology
JF - Canadian Journal of Microbiology
IS - 10
ER -