Bilateral symmetry of linear streptomycete chromosomes

Lis Algora-Gallardo, JANA SCHNIETE, David R Mark, Iain S Hunter, Paul R Herron

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Abstract

Here, we characterize an uncommon set of telomeres from Streptomyces rimosus ATCC 10970, the parental strain of a lineage of one of the earliest-discovered antibiotic producers. Following the closure of its genome sequence, we compared unusual telomeres from this organism with the other five classes of replicon ends found amongst streptomycetes. Closed replicons of streptomycete chromosomes were organized with respect to their phylogeny and physical orientation, which demonstrated that different telomeres were not associated with particular clades and are likely shared amongst different strains by plasmid-driven horizontal gene transfer. Furthermore, we identified a ~50 kb origin island with conserved synteny that is located at the core of all streptomycete chromosomes and forms an axis around which symmetrical chromosome inversions can take place. Despite this chromosomal bilateral symmetry, a bias in parS sites to the right of oriC is maintained across the family Streptomycetaceae and suggests that the formation of ParB/parS nucleoprotein complexes on the right replichore is a conserved feature in streptomycetes. Consequently, our studies reveal novel features of linear bacterial replicons that, through their manipulation, may lead to improvements in growth and productivity of this important industrial group of bacteria.
Original languageEnglish
Article number000692
Pages (from-to)1-13
JournalMicrobial genomics
Volume7
Issue number11
Early online date15 Nov 2021
DOIs
Publication statusPublished - 15 Nov 2021

Keywords

  • bilateral symmetry
  • linear chromosome
  • oriC
  • Streptomyces

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