Antiangiogenic VEGF165b expression in human breast MCF-7 and MCF-10A cells exposed to reverse transcriptase and protease inhibitors,Expresión de VEGF165b Antiangiogénico en Células MCF-7 y MCF-10A de Mama Humana Expuesto a Inhibidores de la Transcriptasa Inversa y la Proteasa

Gbenga Anthony Adefolaju*, Margot Hosie, H. Jill

*Corresponding author for this work

Research output: Contribution to journalArticle (journal)peer-review

4 Citations (Scopus)

Abstract

The combined antiretroviral therapy (cART), a multidrug combination regimen, usually consisting Nucleoside Reverse Transcriptase Inhibitors, non-Nucleoside Reverse Transcriptase Inhibitors and Protease Inhibitors has altered the morbidity pattern affecting HIV-infected individuals to include non-AIDS-defining malignancies (nADMs). The speculation is rife; does cART induce or promote the progression of nADMs such as breast cancer? This study was therefore designed to investigate of the effects of some antiretroviral drugs (at clinically relevant concentrations) on the expression of anti-angiogenic gene; VEGF165b in two human breast cell lines; MCF-7 and MCF-10A by Real Time qPCR and immuno-fluorescence. All of the antiretroviral drugs and combinations tested produced patterns of slight up or downregulation of VEGF165b mRNA expression but the alterations did not attain statistical significance. They also did not alter VEGF165bprotein localisation in both cell lines. The findings reported here suggest that antiretroviral drugs probably do not influence the angiogenic pathway in the development of breast cancer in patients under the combined antiretroviral regimen.

Original languageEnglish
Pages (from-to)148-156
Number of pages9
JournalInternational Journal of Morphology
Volume35
Issue number1
DOIs
Publication statusPublished - 1 Jan 2017

Keywords

  • Breast cancer
  • MCF-10A
  • MCF-7
  • Protease Inhibitors
  • Reverse Transcriptase Inhibitors
  • VEGF165b

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