TY - CHAP
T1 - An Improved In Vitro Porcine Blood-Brain Barrier Model for Permeability Screening and Functional Studies
AU - Patabendige, Adjanie
N1 - Funding Information:
This work was funded by the UK Department for Trade and Industry. Support and useful discussions with Joan Abbott and Rob Skinner during the initial stages of the development of the model is acknowledged. AP also acknowledges support by the NSW Ministry of Health, Australia, under the NSW Health Early-Mid Career Fellowships Scheme and Edge Hill University, UK. All content is solely the responsibility of the author and do not reflect the views of the NSW Health Entity. Some of this work is derived from Patabendige et al. [5] and is used under a Creative Commons Attribution License (https://creativecommons.org/licenses/ by/3.0/). The original version can be found here (https://doi. org/10.1016/j.brainres.2013.04.006).
Publisher Copyright:
© 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2022/6/23
Y1 - 2022/6/23
N2 - The availability of good in vitro blood-brain barrier (BBB) models that closely mimic in vivo BBB features are essential for central nervous system (CNS) drug permeability screening and BBB functionality studies. Of the currently available monoculture primary BBB models, porcine brain endothelial cell models have the best barrier properties, which make them highly suitable for CNS drug permeability screening. In addition, they retain major BBB features such as BBB transporters, receptors, and enzymes and express BBB tight junctions. Therefore, porcine BBB models are also suitable for BBB functionality studies. This paper describes a procedure for extraction of brain microvessels from fresh porcine brains and the culture of pure primary porcine brain endothelial cells. In addition, techniques to improve culture purity and quality, and increase barrier tightness without using co-cultures are given. Using this method, a robust and reproducible in vitro BBB model can be established for CNS permeability screening and studying BBB functionality.
AB - The availability of good in vitro blood-brain barrier (BBB) models that closely mimic in vivo BBB features are essential for central nervous system (CNS) drug permeability screening and BBB functionality studies. Of the currently available monoculture primary BBB models, porcine brain endothelial cell models have the best barrier properties, which make them highly suitable for CNS drug permeability screening. In addition, they retain major BBB features such as BBB transporters, receptors, and enzymes and express BBB tight junctions. Therefore, porcine BBB models are also suitable for BBB functionality studies. This paper describes a procedure for extraction of brain microvessels from fresh porcine brains and the culture of pure primary porcine brain endothelial cells. In addition, techniques to improve culture purity and quality, and increase barrier tightness without using co-cultures are given. Using this method, a robust and reproducible in vitro BBB model can be established for CNS permeability screening and studying BBB functionality.
KW - blood-brain barrier
KW - endothelial cells
KW - In vitro models
KW - CNS drug discovery
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U2 - 10.1007/978-1-0716-2289-6_7
DO - 10.1007/978-1-0716-2289-6_7
M3 - Chapter
C2 - 35733042
SN - 9781071622889
VL - 2492
T3 - Methods in molecular biology (Clifton, N.J.)
SP - 131
EP - 142
BT - The blood-brain barrier
A2 - Stone, N
ER -