A transposon insertion single-gene knockout library and new ordered cosmid library for the model organism Streptomyces coelicolor A3(2)

L. T. Fernández-Martínez, R. Del Sol, M. C. Evans, S. Fielding, P. R. Herron, G. Chandra, P. J. Dyson

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

A simple and high-throughput transposon mediated mutagenesis system employing in vitro shuttle transposon mutagenesis has been used to systematically mutagenise the Streptomyces coelicolor genome. To achieve the highest coverage, a new ordered cosmid library was also constructed. Individual cosmids from both the existing and new libraries were disrupted using the Tn5-based mini-transposon Tn5062. A total of 35,358 insertions were sequenced resulting in the disruption of 6,482 genes (83% of the predicted open reading frames). Complete information for both the newly generated cosmids as well as all the insertions has been uploaded onto a central database, StrepDB (http://​strepdb.​streptomyces.​org.​uk/​). All insertions, new cosmids and a range of transposon exchange cassettes are available for study of individual gene function.
Original languageEnglish
Pages (from-to)515-522
JournalAntonie van Leeuwenhoek
Volume99
Issue number3
DOIs
Publication statusPublished - 2011

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