A new lineage of halophilic, wall-less, contractile bacteria from a brine-filled deep of the Red Sea.

André Antunes, Fred A Rainey, Gerhard Wanner, Marco Taborda, Jürgen Pätzold, M Fernanda Nobre, Milton S da Costa, Robert Huber

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

A novel strictly anaerobic bacterium designated strain SSD-17BT was isolated from the hypersaline brinesediment interface of the Shaban Deep, Red Sea. Cells were pleomorphic but usually consisted of a central coccoid body with one or two “tentacle-like” protrusions. These protrusions actively alternated between a straight, relaxed form and a contracted, corkscrew-like one. A peptidoglycan layer was not detected by electron microscopy. The organism forms “fried-egg”-like colonies on MM-X medium. The organism is strictly anaerobic and halophilic and has an optimum temperature for growth of about 30 to 37°C and an optimum pH of about 7. Nitrate and nitrite are reduced; lactate is a fermentation product. The fatty acid profile is dominated by straight saturated and unsaturated chain compounds. Menaquinone 4 is the major respiratory quinone. Phylogenetic analysis demonstrated strain SSD-17BT represents a novel and distinct lineage within the radiation of the domain Bacteria. The branching position of strain SSD-17BT was equidistant to the taxa considered to be representative lineages of the phyla Firmicutes and Tenericutes (with its sole class Mollicutes). The phenotypic and phylogenetic data clearly show the distinctiveness of this unusual bacterium, and we therefore propose that strain SSD-17BT (! DSM 18853 ! JCM 14575) represents a new genus and a new species, for which we recommend the name Haloplasma contractile gen. nov., sp. nov. We are also of the opinion that the organism represents a new order-level taxon, for which we propose the name Haloplasmatales.
Original languageEnglish
Pages (from-to)3580-3587
JournalJournal of Bacteriology
Volume190
Issue number10
DOIs
Publication statusPublished - May 2008

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Silver Sulfadiazine
Tenericutes
Bacteria
Names
Organism Forms
Anaerobic Bacteria
Peptidoglycan
Nitrites
Nitrates
Fermentation
Ovum
Lactic Acid
Fatty Acids
Temperature
brine
Growth

Cite this

Antunes, A., Rainey, F. A., Wanner, G., Taborda, M., Pätzold, J., Nobre, M. F., ... Huber, R. (2008). A new lineage of halophilic, wall-less, contractile bacteria from a brine-filled deep of the Red Sea. Journal of Bacteriology, 190(10), 3580-3587. https://doi.org/10.1128/JB.01860-07
Antunes, André ; Rainey, Fred A ; Wanner, Gerhard ; Taborda, Marco ; Pätzold, Jürgen ; Nobre, M Fernanda ; da Costa, Milton S ; Huber, Robert. / A new lineage of halophilic, wall-less, contractile bacteria from a brine-filled deep of the Red Sea. In: Journal of Bacteriology. 2008 ; Vol. 190, No. 10. pp. 3580-3587.
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abstract = "A novel strictly anaerobic bacterium designated strain SSD-17BT was isolated from the hypersaline brinesediment interface of the Shaban Deep, Red Sea. Cells were pleomorphic but usually consisted of a central coccoid body with one or two “tentacle-like” protrusions. These protrusions actively alternated between a straight, relaxed form and a contracted, corkscrew-like one. A peptidoglycan layer was not detected by electron microscopy. The organism forms “fried-egg”-like colonies on MM-X medium. The organism is strictly anaerobic and halophilic and has an optimum temperature for growth of about 30 to 37°C and an optimum pH of about 7. Nitrate and nitrite are reduced; lactate is a fermentation product. The fatty acid profile is dominated by straight saturated and unsaturated chain compounds. Menaquinone 4 is the major respiratory quinone. Phylogenetic analysis demonstrated strain SSD-17BT represents a novel and distinct lineage within the radiation of the domain Bacteria. The branching position of strain SSD-17BT was equidistant to the taxa considered to be representative lineages of the phyla Firmicutes and Tenericutes (with its sole class Mollicutes). The phenotypic and phylogenetic data clearly show the distinctiveness of this unusual bacterium, and we therefore propose that strain SSD-17BT (! DSM 18853 ! JCM 14575) represents a new genus and a new species, for which we recommend the name Haloplasma contractile gen. nov., sp. nov. We are also of the opinion that the organism represents a new order-level taxon, for which we propose the name Haloplasmatales.",
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Antunes, A, Rainey, FA, Wanner, G, Taborda, M, Pätzold, J, Nobre, MF, da Costa, MS & Huber, R 2008, 'A new lineage of halophilic, wall-less, contractile bacteria from a brine-filled deep of the Red Sea.', Journal of Bacteriology, vol. 190, no. 10, pp. 3580-3587. https://doi.org/10.1128/JB.01860-07

A new lineage of halophilic, wall-less, contractile bacteria from a brine-filled deep of the Red Sea. / Antunes, André; Rainey, Fred A; Wanner, Gerhard; Taborda, Marco; Pätzold, Jürgen; Nobre, M Fernanda; da Costa, Milton S; Huber, Robert.

In: Journal of Bacteriology, Vol. 190, No. 10, 05.2008, p. 3580-3587.

Research output: Contribution to journalArticle

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T1 - A new lineage of halophilic, wall-less, contractile bacteria from a brine-filled deep of the Red Sea.

AU - Antunes, André

AU - Rainey, Fred A

AU - Wanner, Gerhard

AU - Taborda, Marco

AU - Pätzold, Jürgen

AU - Nobre, M Fernanda

AU - da Costa, Milton S

AU - Huber, Robert

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N2 - A novel strictly anaerobic bacterium designated strain SSD-17BT was isolated from the hypersaline brinesediment interface of the Shaban Deep, Red Sea. Cells were pleomorphic but usually consisted of a central coccoid body with one or two “tentacle-like” protrusions. These protrusions actively alternated between a straight, relaxed form and a contracted, corkscrew-like one. A peptidoglycan layer was not detected by electron microscopy. The organism forms “fried-egg”-like colonies on MM-X medium. The organism is strictly anaerobic and halophilic and has an optimum temperature for growth of about 30 to 37°C and an optimum pH of about 7. Nitrate and nitrite are reduced; lactate is a fermentation product. The fatty acid profile is dominated by straight saturated and unsaturated chain compounds. Menaquinone 4 is the major respiratory quinone. Phylogenetic analysis demonstrated strain SSD-17BT represents a novel and distinct lineage within the radiation of the domain Bacteria. The branching position of strain SSD-17BT was equidistant to the taxa considered to be representative lineages of the phyla Firmicutes and Tenericutes (with its sole class Mollicutes). The phenotypic and phylogenetic data clearly show the distinctiveness of this unusual bacterium, and we therefore propose that strain SSD-17BT (! DSM 18853 ! JCM 14575) represents a new genus and a new species, for which we recommend the name Haloplasma contractile gen. nov., sp. nov. We are also of the opinion that the organism represents a new order-level taxon, for which we propose the name Haloplasmatales.

AB - A novel strictly anaerobic bacterium designated strain SSD-17BT was isolated from the hypersaline brinesediment interface of the Shaban Deep, Red Sea. Cells were pleomorphic but usually consisted of a central coccoid body with one or two “tentacle-like” protrusions. These protrusions actively alternated between a straight, relaxed form and a contracted, corkscrew-like one. A peptidoglycan layer was not detected by electron microscopy. The organism forms “fried-egg”-like colonies on MM-X medium. The organism is strictly anaerobic and halophilic and has an optimum temperature for growth of about 30 to 37°C and an optimum pH of about 7. Nitrate and nitrite are reduced; lactate is a fermentation product. The fatty acid profile is dominated by straight saturated and unsaturated chain compounds. Menaquinone 4 is the major respiratory quinone. Phylogenetic analysis demonstrated strain SSD-17BT represents a novel and distinct lineage within the radiation of the domain Bacteria. The branching position of strain SSD-17BT was equidistant to the taxa considered to be representative lineages of the phyla Firmicutes and Tenericutes (with its sole class Mollicutes). The phenotypic and phylogenetic data clearly show the distinctiveness of this unusual bacterium, and we therefore propose that strain SSD-17BT (! DSM 18853 ! JCM 14575) represents a new genus and a new species, for which we recommend the name Haloplasma contractile gen. nov., sp. nov. We are also of the opinion that the organism represents a new order-level taxon, for which we propose the name Haloplasmatales.

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DO - 10.1128/JB.01860-07

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